34Yellow-marginedBoxTurI本文件按照GB/T1.1—2020《標準化工作導則第1部分：標準化文件的結構和起草規則》的規定增、方國俠、沈保平、高凌、徐麗、彭步旭、鮑鳴、張四剛1黃緣閉殼龜本文件給出了黃緣閉殼龜（CuoraflavomarginataGray，1863）的名稱與分類、主要形態構造特征、生長和繁殖特性、遺傳學特征、檢測方法GB/T18654.2養殖魚類種質檢GB/T18654.12養殖魚類種質檢驗GB/T18654.15養殖魚類種質檢驗第4名稱與分類4.2別名4.3分類5.1.1外形體呈橢圓形，前部略窄、后部略寬。頭光滑無鱗，鼓膜圓而清晰，頭部背面呈橄欖色，兩眼后各25.1.2.1背甲35.1.2.3g，背甲長104.6mm～121.2mm，年齡4+～6+。各注：各可量性狀說明參長，胃分化明顯，十二指腸上附有條形白色胰臟，無盲腸。雌雄異體，雄性睪丸1對，淡白色，卵圓形，位于腎臟前端內側，后端為輸精管，通泄殖腔，陰莖位于尾基部的泄殖孔內；雌體卵巢1對，位4于腎臟前端內側，緊靠椎板，呈黃色片狀，內有粒狀卵子，產卵期具多個6.1生長不同年齡組的黃緣閉殼龜體長與體重的實測值變表2黃緣閉殼龜不同年齡組實測背甲長+注：生長方程和背甲長與體重關系式參加附錄A6.2繁殖7.1.1體細胞染色體數：2n=52；染色體臂數（NF）：70；核型公式57.2分子遺傳學特征黃緣閉殼龜線粒體16SrRNA基因片段的堿基序列為1596bp％，8.2性狀測定8.3年齡鑒定8.4細胞遺傳學檢測69判定規則7Wt1=12.73e0.476（r=0.9843）·················································Lt=45.47T0.466（r=0.9944）········································Wt2=34.81T0.461（r=0.9939）·····················································(A.3)Wt2——t齢時龜背甲寬，單位為毫米（mmWt1=Lt3.018（r=0.9899）··························································(Wt1——t齢時龜體重，單位為克（gLt=0.82Wt21.191（r=0.9925）·············································Wt2——t齢時龜背甲寬，單位為毫米（mm8取黃緣閉殼龜肌肉組織，剪碎，10％蛋白酶K消化，DNA提取試劑盒B.2.1擴增引物序列為16SF:5'-CACAATAAACAAACAGATGAGGC取5μL擴增產物上樣到1.0％的瓊脂糖凝膠進行電泳分），利用Kimura兩參數模型（Kimura2param9黃緣閉殼龜線粒體16SrRNA基因標準序列（1596b1GCAAGAACCTAGCCCAACCAAAATAAAAAACTTAACAAACAAAACTATCC51CACCAAAACCAACCAAAACATTCTCACCATCCTAGTATAGGCGATAGAAA101AGACAGTTGGAGCAATAGAGATAGTACCGCAAGGGAAAGATGAAAAACAA151TGAAATATTCACTAAGCCATAAAAAGCAAAGATTAATCCTTATACCTTTT201GCATCATGATTTAGCTAGCATATTCAAGCAAAGAGCCCTAAAGTATGAAA251CCCCGAAACTAAGTGAGCTACTTAAAGGCAGCCACACCAAGGCTAAATCC301GTCTCTGTGGCAAAAGAGTGGAAAGACCTGTAAGTAGAGGTGAAAAGCCT351AACGAACTTAGTGATAGCTGGTTGCTCAATAAAAGAATATAAGTTCAACC401TTAAACCTTCTAAAAACAACCTAAAGTATAAAAGAAAAGTTTAAGATTTA451TTCAATTGGGGTACAGCCCAATTGAAAAAGGATACAACCAATAACGGAGG501ACAAAACCCCAAAAATAACAACCGTAGGCCTTAAAGCAGCCACCAACTAA551GAAAGCGTCAAAGCCCACCCGCTATTAAATAGTAACAAAAAATTTTTTCC601CCTAATAATATTGAGCTACTCCATTTAAATAGAGGAATTAATGCTAAAAT651GAGTAACAAGAAGACAAAACTTCTCTAATACGCTAGCTTAAGTCATAACA701GATAAACTACTGACTATTAACATCCATCACTATAAAACAACAACATTTTA751AATCACCCTATAGACCTAACTGTTAACCCAACACAGGAGCGTATACAAGA801AAGATTAAAATTTGTAAAAGGAACTAGGCAAATAAACGAGCCCGACTGTT851TACCAAAAACATAGCCCCTAGCAACACAAGTATTAGGGGTAATGCCTGCC901CAGTGACACTGTTAAACGGCCGCGGTATCCTAACCGTGCAAAGGTAGCGT951AATCACTTGTCTTTTAAATAAAGACTAGAATGAATGGCCAAACGAGGTTC1001TACCTGTCTCTTACAAATAATCAGTGAAATTGATCTTCCTGTGCAAAAGC1051AGGAATAACATTATAAGACGAGAAGACCCTGTGGAACTTCAAATACAAAT1101CAACTATTATCAACACCCACCTACGGGTCCACATCAAACTAGCATCTGAC1151TTATATTTTCGGTTGGGGCGACCTCGGAGTAAAATAAAACCTCCGAAAAA1201GAATTTCCTTTTAAACCTAGACCTACAGCCCACAGTGCTTCCAGCAAAAC1251GATCCAATATACTTGATCAACGAACCAAGCTACCCCAGGGATAACAGCGC1301AATCCCATCCTAGAGTTCCTATCGACGATGGAGTTTACGACCTCGATGTT1351GGATCAGGACATCCTGATGGTGCAACTGCTATCAAGGGTTCGTTTGTTCA1401ACGATTAACAGTCCTACGTGATCTGAGTTCAGACCGGAGCAATCCAGGTC1451