Hotline:400-820-3792Inhibitors?Agonists?ScreeningLibrarieswww.MedChemED-LuciferinpotassiumsaltCat.No.:HY-12591BCASNo.:115144-35-9分?式:C??H?KN?O?S?分?量:318.41作?靶點(diǎn):Others作?通路:Others儲(chǔ)存?式:4°C,protectfromlight*Insolvent:-80°C,6months;-20°C,1month(protectfromlight)溶解性數(shù)據(jù)體外實(shí)驗(yàn)H2O:25mg/mL(78.52mM;Needultrasonic)掃描?維碼，運(yùn)?溶解?案計(jì)算器獲得適合您實(shí)驗(yàn)體系的溶解?案MassSolvent1mg5mg10mgConcentration制備儲(chǔ)備液1mM3.1406mL15.7030mL31.4060mL5mM0.6281mL3.1406mL6.2812mL10mM0.3141mL1.5703mL3.1406mL請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度，選擇合適的溶劑配制儲(chǔ)備液，并請(qǐng)注意儲(chǔ)備液的保存?式和期限。BIOLOGICALACTIVITY?物活性D-Luciferinpotassiumsalt螢光素酶的底物，可催化?物發(fā)光昆?產(chǎn)?典型的綠光[1]。體外研究D-luciferinisthenaturalsubstrateoftheenzymeluciferase(Luc),thatcatalyzestheproductionofthetypicalyellowgreenlightoffireflies.ThepresentreviewcoversthesynthesisofD-luciferinandderivativesoranaloguesthataresubstratesorinhibitorsoftheluciferasefromtheAmericanfireflyPhotinuspyralis,theenzymemorefrequentlyusedintechniquesofinvitroandopticalimaging[1].D-LuciferinexhibitsadecreaseinthemeasuredKminPC3M-LuccelllysateswithaKmof34μM[2].體內(nèi)研究Bioluminescenceimaging(BLI)usingthefireflyluciferase(Fluc)asareportergeneandD-luciferinasa1/3www.MedChemEwww.MedChemEsubstrateiscurrentlythemostwidelyemployedtechnique.ThetotalsignalintensityisplottedagainstthetimeafterD-luciferininjectiontogenerateatime-intensitycurve.Inadditiontothepeaksignal,thesignalsatfixedtimepoints(5,10,15,and20?min)afterD-luciferininjectionaredeterminedasalternativestothepeaksignal.Thesignalinagiventime-intensitycurveisnormalizedforthepeaksignalinthecurvetorepresentthepatternoftemporalchangesafterD-luciferininjection[3].Injectwith10μLofD-luciferin(intraperitoneallyorintravenously)stocksolutionpergramofbodyweight:normally~200μLfora20gmouseforastandard150mg/kginjection.ThawD-Luciferin(eitherPotassiumorSodiumSalt)atroomtemperatureanddissolveindPBS(nocalciumormagnesium)toafinalconcentrationof15mg/mL.Pre-weta0.22μmfilterbydrawingthrough5-10mLofsterileH2Oanddiscardwater.SterilizetheD-Luciferinsolutionthroughtheprepared0.22μmsyringefilter.PROTOCOLAnimalMice[2]Administration[2]InvivoBLIisperformedusingacooledcharge-coupleddevicecamerasystem(IVISImagingSystem100)3,5,7,10,12,14,19,21,24,and28daysaftertheinoculationofHCT116-Luccells.Miceareinjectedwith75?mg/kgD-luciferinin100?μLofphosphate-bufferedsalinesubcutaneously.Beginning5?minafterinjection,dorsalluminescentimageswithanexposuretimeof1?sareacquiredsequentiallyatarateofoneimageperminuntil20?minafterD-luciferininjection.Dataacquisitioniscontinueduntil40?minpostinjectionondays3or5anduntil25?minonday7,becauseoftheprolongedtimecourseoflightemission.Binningis4andthefieldofviewis15?cm.MCEhasnotindependentlyconfirmedtheaccuracyofthesemethods.Theyareforreferenceonly.戶使?本產(chǎn)品發(fā)表的科研?獻(xiàn)?CellDeathDis.2020Sep17;11(9):765.?PharmacolRes.2021Mar2;105527.?BiomedPharmacother.2019Sep;117:109126.?IntJOncol.2020Mar;56(3):761-771.?JLeukocBiol.2019Nov;106(5):1089-1100.Seemorecustomervalidationsonwww.MedChemEREFERENCES[1].GiuseppeMeroni,etal.D-Luciferin,derivativesandanalogues:synthesisandinvitro/invivoluciferase-catalyzedbioluminescentactivity.ARKIVOC2009(i)265-288.[2].InoueY,etal.Timingofimagingafterd-luciferininjectionaffectsthelongitudinalassessmentoftumorgrowthusinginvivobioluminescenceimaging.IntJBiomedImaging.2010;2010:471408.[3].RajeshShinde,etal.Luciferinderivativesforenhancedinvitroandinvivobioluminescenceassays.Biochemistry.2006Sep19;45(37):11103-12.McePdfHeight2/3www.MedChemEwww.MedChemE關(guān)注MCE中國(guó)公眾號(hào)，

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