1、限制性酶的識別序列Enzyme Name Sequence AatI AGGCCT AatII GACGTC AccI GTMKAC AccII CGCG AccIII TCCGGA AcyI GRCGYC AflI GGWCC AflII CTTAAG AflIII ACRYGT AhaI CCSGG AhaII GRCGYC AhaIII TTTAAA AluI AGCT AlwI GGATCNNNN AlwI NNNNNGATCC AlwNI CAGNNNCTG AocI CCTNAGG AocII GDGCHC AosI TGCGCA AosII GRCGYC ApaI GGGCCC

2、ApaLI GTGCAC ApyI CCWGG AquI CYCGRG AseI ATTAAT AspI GAANNNNTTC AspI GGTACC AspI GACNNNGTC AsuI GGNCC AsuII TTCGAA AvaI CYCGRG AvaII GGWCC AvaIII ATGCAT AvrI CYCGRG AvrII CCTAGG AxyI CCTNAGG BalI TGGCCA BamHI GGATCC BanI GGYRCC BanII GRGCYC BanIII ATCGAT BbeI GGCGCC BbiII/AcyI GRCGYC BbvI GCAGCNNNNN

3、NNN BbvI NNNNNNNNNNNNGCTGC BbvII GAAGACNN BbvII NNNNNNGTCTTC BcefI ACGGCNNNNNNNNNNNN BcefI NNNNNNNNNNNNNGCCGT BclI TGATCA BcnI CCSGG BglI GCCNNNNNGGC BglII AGATCT BinI NNNNNGATCC BinI GGATCNNNN BsePI GCGCGC BsmAI GTCTC BsmAI GAGAC BsmI GAATGCN BsmI GCATTC BspI GDGCHC BspHI TCATGA BspMI ACCTGCNNNN Bs

4、pMI NNNNNNNNGCAGGT BspMII TCCGGA BsrI ACTGGN BsrI CCAGT BssHII GCGCGC BstBI TTCGAA BstEII GGTNACC BstI GGATCC BstNI CCWGG BstPI GGTNACC BstUI CGCG BstXI CCANNNNNNTGG BstYI RGATCY BsuI CCTNAGG CcrI CTCGAG CfoI GCGC CfrI RCCGGY CfrI GGNCC CfrI YGGCCR ClaI ATCGAT CviJI RGCY CvnI CCTNAGG DdeI CTNAG DpnI

5、 GATC DraI TTTAAA DraII RGGNCCY DraIII CACNNNGTG DsaI CCRYGG EaeI YGGCCR EagI CGGCCG EarI CTCTTC EarI GAAGAG EclXI CGGCCG EcoI TACGTA EcoI GGTCTCN EcoI NNNNNGAGACC EcoI GGWCC EcoIII AGCGCT EcoI CGGCCG EcoI CTGAAG EcoI CTTCAG EcoI CCTNAGG EcoNI CCTNNNNNAGG EcoOI RGGNCCY EcoRI GAATTC EcoRII CCWGG EcoR

6、V GATATC EcoTI CCWWGG EcoTI ATGCAT EcoTI GRGCYC EheI GGCGCC EspI GCTNAGC FinI GTCCC FinI GGGAC FnuHI GCNGC FokI GGATGNNNNNNNNN FokI NNNNNNNNNNNNNCATCC FspI TGCGCA GdiII NNNNNYGGCCG GdiII CGGCCRN GsuI CTCCAG GsuI CTGGAG HaeI WGGCCW HaeII RGCGCY HaeIII GGCC HapII CCGG HgaI GACGCNNNNN HgaI NNNNNNNNNNGC

7、GTC HgiAI GWGCWC HgiEII ACCNNNNNNGGT HhaI GCGC HinI GRCGYC HinPI GCGC HincII GTYRAC HindIII AAGCTT HinfI GANTC HpaI GTTAAC HpaII CCGG HphI GGTGANNNNNNNN HphI NNNNNNNTCACC KpnI GGTACC KspI CTCTTCN KspI NNNNGAAGAG MaeI CTAG MaeII ACGT MaeIII GTNAC MboI GATC MboII GAAGANNNNNNNN MboII NNNNNNNTCTTC MfeI

8、CAATTG MflI RGATCY MluI ACGCGT MmeI TCCRAC MmeI GTYGGA MnlI CCTCNNNNNNN MnlI NNNNNNNGAGG MroI TCCGGA MseI TTAA MspI CCGG MstI TGCGCA MstII CCTNAGG MvaI CCWGG NaeI GCCGGC NarI GGCGCC NciI CCSGG NcoI CCATGG NdeI CATATG NdeII GATC NheI GCTAGC NlaIII CATG NlaIV GGNNCC NotI GCGGCCGC NruI TCGCGA NsiI ATGC

9、AT Nsp()I RCATGY Nsp()V TTCGAA NspBII CMGCKG NspII GDGCHC NspIII CYCGRG NspIV GGNCC NunII GGCGCC PaeR CTCGAG PalI GGCC PflMI CCANNNNNTGG PleI GAGTCNNNN PleI NNNNNGACTC PmaCI CACGTG PpuMI RGGWCCY PstI CTGCAG PvuI CGATCG PvuII CAGCTG RsaI GTAC RsrI GAATTC RsrII CGGWCCG SacI GAGCTC SacII CCGCGG SalI GT

10、CGAC SauAI GATC SauI GGNCC SauI CCTNAGG ScaI AGTACT ScrFI CCNGG SduI GDGCHC SecI CCNNGG SexI CTCGAG SfaNI GCATCNNNNN SfaNI NNNNNNNNNGATGC SfiI GGCCNNNNNGGCC SinI GGWCC SmaI CCCGGG SnaBI TACGTA SnaI GTATAC SpeI ACTAGT SphI GCATGC SplI CGTACG SspI AATATT SstI GAGCTC SstII CCGCGG SstIII ACGT StuI AGGCCT StyI CCWWGG StySJI GAGNNNNNNGTRC StySJI GYACNNNNNNCTC TaqI TCGA TaqII GACCGANNNNNNNNNNN TaqII NNNNNNNNNTCGGTC TaqII CACCCANNNNNNNNNNN TaqII NNNNNNNNNTGGGTG ThaI CGCG TspI GTSAC TspEI AATT TthI GACNNNGTC TthII