大鼠下丘腦室旁核內一氧化氮合酶神經元的生后發育解剖科學進展 2000年第3期第6卷 原著作者：王蘭 宋天保 周勁松單位：王蘭（陜西省高等醫學專科學校解剖教研窒 西安 710068）；宋天保 周勁松（西安醫科大學組織與胚胎學教研室）關鍵詞：一氧化氮合酶；視上核；室旁核；生后發育；NADPH-黃遞酶組織化學；免疫組織化學；大鼠摘要：應用NADPH-黃遞酶組織化學和一氧化氮合酶(NOS)免疫組織化學方法以及計算機圖像分析，觀察NOS神經元在大鼠下丘腦室旁核(PVN)生后發育各階段的形態及分布特征。結果顯示，1 d時PVN內已有NOS神經元。隨著生長發育，PVN的面積逐漸變化，NOS神經元主要集中在PVN的外側大細胞部及腹側部，細胞逐漸增多，胞體的平均截面積逐漸變大，平均灰度值逐漸降低。14 d以后至成年鼠(90 d)以上變化不明顯。結果提示，PVN中NOS神經元的生后發育和成熟主要在14 d前，尤以7 d至14 d為關鍵時期。Postnatal Development of NOS Neurons in the Paraventricular Nucleus of Rat Hypothalamus wang LanAbstract：In the present research, the postnatal development of nitric oxide synthase(NOS) neurons in the hypothalamic paraventricular nucleus (PVN) of the rat was studied by means of nADPH-d histochemical, NOS immunohistochemical methods and image analysis. The results showed that NOS neurons were present in PVN on day 1 of postnatal life. With the development, the PVN was gradually enlarged in size and NOS neurons were predominantly confined within lateral magnocellular and ventral subdivisions of pVN. NOS neurons were increased in number and the soma area was increased while grey level decreased. The number and morphology of NOS neurons remained unchanged after day 14. These results indicate that the main developmental stage of NOS neurons is from d 1 to d 14, especially from d 7 to d 14.Keywords：nitric oxide synthase; paraventricular nucleus; postnatal development; NADPH-d histochemical; immunohistochemical; rat參考文獻：1鐘慈聲，孫安陽一氧化氮的生物醫學上海上海醫科大學出版社，1997; 162Hope BT, Micheal GJ, Kingge KM, et al. Neuronal NADPHdiaphorase is a nitric oxide synthase. Proc Nad Acad SCi USA, 1991, 88: 28113Areralo R, Sanchez F, Alonso JR, et al. NADPH-diaphorase activity in the hypothalamic magnocellular, neurosecretory nuclei of the rat. Brain Res Bull, 1992, 28: 5994Aimi Y, Fujimura M, Vincent SR, et al. Localization of NADPH-diaphorase-containing neurons in sensory gangem of the rat. J Comp neurol, 1991, 306: 3825Vahschanoff JG, Weinberg RJ, Rustioni A. NADPH-diaphorase in the spinal cord of rats. J Comp neurol, 1992, 321: 2O96賈鈾生，鞠躬神經系統中的一氧化氮合酶神經解剖學雜志，1993，9: 17王百忍，趙經緯一氧化氮合酶與痛神經解剖學雜志，1995，11: 698倪衡建，凌樹才，萬人欣，等大鼠下丘腦室旁核中一氧化氮合酶陽性神經元的生后發育神經解剖學雜志，1997，13(2): 1719朱長庚，張喜京，劉漢濤，等室旁核膽囊收縮素神經元的電鏡免疫細胞化學研究解剖學報，1990，21: 24810Okamura H, Yokosuka M, Hayashi S. Estrogenic induction of NADPH-diaphorase activity in the prcoptic neurons containing estrogen recepto