1、靈芝煎劑對亞稀褶黑菇急性中毒大鼠腎臟損傷保護作用的研究 09-01-30 09:29:00 編輯：studa20 作者：肖宮，熊軍，羅崇岱，楊可達，張平 肖桂林【摘要】 目的 探討靈芝煎劑對亞稀褶黑菇急性中毒大鼠腎臟的保護作用。方法 用SD大鼠20只，隨機分為4組，分別為：空白組，采用生理鹽水灌胃對照；模型組，采用亞稀褶黑菇毒素灌胃造模；模型組，采用亞稀褶黑菇毒素灌胃造模后，用靈芝煎劑灌胃治療；靈芝組，采用靈芝煎劑灌胃對照。觀察大鼠的存活情況、活動情況、尿顏色、尿量、尿紅細胞、尿白細胞、尿蛋白、血液生化（尿素氮、肌酐）、NAG酶以及腎臟病理檢查等指標。結果 實驗所觀測的指標包括實驗動物的存活情

2、況、活動情況、尿顏色、尿量、尿紅細胞、尿白細胞、尿蛋白、血液生化（尿素氮、肌酐）、NAG酶等，模型組與模型組各項指標分別比較，有非常顯著差異（P0.01）；病理形態學檢查結果提示，模型組的腎臟組織結構損害較模型組明顯減輕。結論 靈芝煎劑對亞稀褶黑菇中毒大鼠腎臟有明顯的保護作用。 【關鍵詞】 靈芝煎劑；亞稀褶黑菇；SD大鼠；急性中毒；腎臟保護 Protective efficacy of Lingzhi decoction on acute renal injury caused by russula subnigricans in rats XIAO Gong, XIONG Jun, LUO

3、Chong-dai, YANG Ke-da, ZHANG Ping, XIAO Gui-lin (Xiangya medical college, Central South University, Changsha, Hunan 410008, China) To investigate the protective efficacy of Lingzhi decoction on acute kidney injury caused by russula subnigricans in rats. Methods 20 female SD rats with russula subnigr

4、icans were ramdomly divided into 4 groups as follows, normal group, fed with normal saline, model group, with russula subnigricans, model group, with Lingzhi decoction after modeled by russula subnigricans, and fourth group, with Lingzhi decoction only for comparison. Results All the indexes of livi

5、ng condition, behavior, color and volume of urine, RBC, WBC, protein, blood biochemistry(UN, Cr) and -N-acetyglocosamidase NAG in model group were very different compared with model group(P0.01) ; the damages of the cells and tissues of kidney in model group were apparently much lighter than that in

6、 model group with pathological examination. Conclusions The Lingzhi decoction is very effective to protect rats kidney damaged by russula subnigricans. Lingzhi decoction; russula subnigricans; SD rats; acute poisoning ; protecting kidney 在指導老師臨床治療的基礎上1，2，為進一步探索靈芝對亞稀褶黑菇中毒腎臟的保護作用，采用亞稀褶黑菇模擬人中毒過程，用大鼠制造中毒動物模型，然后用靈芝煎劑灌胃進行治療，觀察腎臟功能及病理變化情況，以了解靈芝煎劑對亞稀褶黑菇中毒大鼠腎臟的保護作用，驗證靈芝對亞稀褶黑菇中毒病人臨床解毒作用。 1 材料與方法 1.1 材料 1.2 方法 1.3 觀測指標 1.4 統計學分析 數據采用SPSS11.5統計軟件分析處理，計量資料用均數標準差（）表示，多組間比較用方差分析。 2 結果 2.1 靈芝煎劑對亞稀褶黑菇急性中毒大鼠存活的影響 模型組于實驗48 h 以后死亡2只，其余所有實驗動物至實驗結束。 2.2 靈芝煎劑對亞稀褶黑菇急性中毒大鼠食量、活動、小便顏色積分的影響 食