1、紅茶提取物茶多酚產(chǎn)品規(guī)格： 30%,60% 茶多酚檢測方法： Folin D法有效成分： 茶多酚產(chǎn)品來源： 山茶科植物山茶的葉提取方法： 乙醇提取產(chǎn)品外觀： 棕黃色粉末1. 理化性質(zhì)分子結(jié)構(gòu)類型：一類多酚化合物溶解性：易溶于水及乙醇，味苦澀2. 質(zhì)量標(biāo)準(zhǔn)檢驗2.1 鑒別：（對照品：沒食子酸）2.1.1 TLC檢查，與對照品Rf值、顏色一致2.1.2 IR檢查，與對照品指紋圖譜吻合（紅外吸收光譜）2.2 紅茶茶多酚的檢測方法：2.2.1 原理福林酚試劑氧化茶多酚中-OH基團(tuán)并顯藍(lán)色，最大吸收波長為765nm， 用沒食子酸作校正標(biāo)準(zhǔn)定量茶多酚。2.2.2 試劑及設(shè)備1. 色譜純乙腈2. 甲醇1.

2、甲醇水溶液（體積比）=7:32. 碳酸鈉(Na2CO3)3. 福林酚試劑4. 10%福林酚試劑（現(xiàn)配）：將20mL福林酚試劑轉(zhuǎn)移到200mL容量瓶中，用水定容并搖勻。5. 7.5% Na2CO3溶液（質(zhì)量濃度）：稱取37.50g0.01gNa2CO3（10.3），加適量水溶解，轉(zhuǎn)移至500mL容量瓶中，定容至刻度，搖勻。6. 沒食子酸標(biāo)準(zhǔn)儲備溶液(1000g/mL): 稱取0.110g0.001g沒食子酸(GA,相對分子質(zhì)量188.14)，于100mL容量瓶中溶解并定容至刻度，搖勻.7. 沒食子酸工作液：用移液管分別移取1.0，2.0，3.0，4.0，5.0mL的沒食子酸標(biāo)準(zhǔn)儲備溶液(10.8

3、)于100mL容量瓶中，分別用水定容至刻度，搖勻，濃度分別為10，20，30，40，50g/mL。2.2.3 樣品液配置準(zhǔn)確稱取10g紅茶提取物樣品，用適量水溶解，轉(zhuǎn)入100mL容量瓶中，加水定容至刻度，搖勻，待測。 2.2.4 樣品含量的檢測用移液管分別取沒食子酸工作液、水及測試液各1.0mL于刻度試管內(nèi)，在每個試管內(nèi)分別加入5.0mL的10%福林酚試劑，搖勻。反應(yīng)3min8min內(nèi)，加入4.0mL7.5% Na2CO3溶液，加水定容至刻度、搖勻。室溫下放置60min。用10mm比色皿、在765nm波長條件下用分光光度計測定吸光度（A）。根據(jù)沒食子酸工作液的吸光度（A）與各工作溶液的沒食子酸

4、濃度，制作標(biāo)準(zhǔn)曲線。 2.2.5 結(jié)果計算比較試樣和標(biāo)準(zhǔn)工作液的吸光度，按下式計算：式中：A樣品測試液吸光度；V樣品液體積；d稀釋因子(稀釋倍數(shù))；SLOPEstd 沒食子酸標(biāo)準(zhǔn)曲線的斜率；m樣品干物質(zhì)含量，%；m1樣品質(zhì)量，單位為克(g)。2.3 檢查：干燥失重： 2%灰分： 0.5%重金屬： 百萬分之五農(nóng)藥殘留： 百萬分之二溶劑殘留：0.1%2.4 衛(wèi)生檢查細(xì)菌總數(shù)：1000/g霉菌總數(shù)：100/g大腸桿菌： 無沙門菌： 無2.5 粒度： 均勻接近細(xì)微顆粒2.6 包裝： 雙層朔料袋封口、外包裝紙桶、桶外印有品名、毛重、批號、生產(chǎn)單位、生產(chǎn)時間、有效時間、貯運方法等。2.7 有效期： 2年2

5、.8 貯藏： 陰涼、干燥、避光貯放3. 提取工藝流程圖紅茶提取物茶多酚的生產(chǎn)工藝紅茶原料清水浸泡乙醇提取殘渣 提取液 過濾 濃縮 質(zhì)量分析和評價 高壓高溫瞬時滅菌 噴霧干燥 篩分 產(chǎn)品包裝 茶多酚4. 描述基本信息：茶多酚又稱茶鞣質(zhì)或茶單寧，是茶葉中多酚類物質(zhì)的總稱，也是形成茶葉色香味及起保健功效的主要成分之一。由于多酚結(jié)構(gòu)的存在，茶多酚易氧化，可用于清除體內(nèi)過剩的自由基、阻止脂質(zhì)過氧化，提高機(jī)體免疫力，延緩衰老等方面。茶多酚等活性物質(zhì)具解毒和抗輻射作用，能有效地阻止放射性物質(zhì)侵入骨髓，并可使鍶90和鈷60迅速排出體外，被健康及醫(yī)學(xué)界譽為“輻射克星”。 喝茶保健養(yǎng)生已經(jīng)成為世界性的健康理念。茶

6、多酚的主要功效： 1 具有很強(qiáng)的消除有害自由基的作用。2 抗衰老作用。3 抗輻射作用。4 對癌細(xì)胞的抑制作用。5 抗菌、殺菌作用。6 對艾滋病病毒的抑制作用。5. 產(chǎn)品導(dǎo)向：美容、減肥、保健品、藥品、保鮮劑6. 安全性及最小用量: 安全Black Tea Extract PolyphenolsProduct Specification: 30%,60% PolyphenolsTest method: Folin DChemical Name: PolyphenolsRaw material: Leaf of Camellia sinensis O. Ktze.Extract Method: G

7、rain AlcoholProduct Type: Brown yellow powder1. Physical and Chemical PropertiesMolecular structure of type: a series Polyphenol compoundsSolubility: Easy dissolve in water and ethanol.2. Quality standards testing2.1 Identification: (reference substance: Gallic acid )2.1.2 TLC examination: reference

8、 substance Rf value and color are consistency2.1.3 IR inspection: in line with the reference substance fingerprint (infrared absorption spectra)2.2 Folin-Ciocalteu method to test Ployphenol in Black Tea Extract2.2.1 TheoryFolin-Ciocalteu solvent can oxidant the OH group in ployphenol and show blue c

9、olor, the m is 765nm; to measure the concentration of ployphenol by using Gallic acid as calibrations.2.2.2 Reagents and equipment1. Chromatography grade Acetonitrile 2. Methanol3. Methanol-Water = 7:3 solvent4. Na2CO3 solvent5. Folin-Ciocalteu solvent6. 10% Folin-Ciocalteu (fresh): 20mL Folin-Cioca

10、lteu solvent be transfer to 200mL volumetric flask, and mix with water to volume.7. 7.5% Na2CO3(weight) solution: accurately takeing 37.50g0.01gNa2CO3 dissolve in water, then transfer to 500mL volumetric flask, and mix with water to volume.8. Gallic acid standard store solution: accurately takeing 0

11、.110g0.001g Gallic acid( GA, MW:188.14) into 100mL volumetric flask, and mix with water to volume.9. Gallic acid working solution: accurately transfer 1.0, 2.0，3.0，4.0，5.0mL Gallic acid standard store solution into 100mL volumetric flask, and mix with water to volume. The concentration series are 10

12、, 20, 30, 40, 50g/mL.2.2.3 Preparation for test sampleAccurately taking 10g of Black Tea Extract samples dissolved in appropriate water, then transfer into 100mL volumetric flask, adding water to volume.2.2.4 Sample content testPartly taking 1.0mL Gallic acid working solution, Water, Test sample add

13、 into 3 graduated test tube. Each test tube be add into 5.0mL 10% Folin-Ciocalteu solvent, mixed. Let the liquid reaction 3min8min, then add 4.0mL 7.5% Na2CO3 solution and water to volume, mixed. Lay at room temperature. Detect the absorbance(A) by a 10mm cuvette at 765nm wave.Making the working cur

14、ve according to the absorbance and the concentration of the Gallic acid.2.2.5 Calculate According to the absorbance(A), calculate the ployphenol as follow formula:Label: Athe absorbance of the test sampleVthe volume of the test sampleddilution factorSLOPEstd the slope of the Gallic acid working curv

15、emweight of dried sample(%)m1weight of sample(g)2.3 ExamineLoss on Drying: NMT 2%Ash: NMT 0.5%Heavy Metals: NMT 5 ppmPesticide Residues: NMT 2 ppmSolvents Residues: NMT 0.1%2.4 Hygiene inspectionTotal bacteria: NMT 1000/gTotal Streptomyces: NMT 100/gE.Coli: NoneSalmonella: None2.5 Sieve Analysis: 10

16、0% pass 80mesh2.6 Packing: Packed in paper-drums and two plastic-bags inside. Barrels outside printed name, gross weight, batch number, production units, production time, effective time, storage and transportation methods.2.7 Effective time: two years Storage situation: Stored in a cool and dry well

17、-closed container, keep away from moisture and strong light / heat.3. Extraction Process Flow DiagramManufacturing Process of Black Tea Extract Ployphenol Black TeaSoak with waterExtract with alcohol Residue Extract solution Filter the extract solution Concentration Quality analysis and evaluation S

18、terilization using high pressure and high temperature for 510 Second Spray drying Sift the powdered extract Packing the powder Ployphenol4. Basic information: Tea health has become a worldwide health concepts. Tea polyphenols, also call tea tannin, is a whole name of a series polyphenols in tea; it is also the main ingredients in tea which shape the special flavour of smell, taste and Color of Solution, also is the main ingredients with health care activity. Due to the polyphenol structure, tea ployphenol is