1、pEASY-Blunt Complete sequenceAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTAGCTCACTCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGCCAAGCTTGGTACCGAGCTCGGATCCACTAGTAACGGCCG

2、CCAGTGTGCTGGAATTGCCCTTAAGGGCAATTCTGCAGATATCCATCACACTGGCGGCCGCTCGAGCATGCATCTAGAGGGCCCAATTCGCCCTATAGTGAGTCGTATTACAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGACGCGCCCTGTAGCGGCGCATTAAGCG

3、CGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACAC

4、TCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATTCAGGGCGCAAGGGCTGCTAAAGGAAGCGGAACACGTAGAAAGCCAGTCCGCAGAAACGGTGCTGACCCCGGATGAATGTCAGCTACTGGGCTATCTGGACAAGGGAAAACGCAAGCGCAAAGAGAAAGCAGGTAGCTTGCAGTGGGCTTACATGGCGATAGCTAGACTGGGCGGTTTTATGGACAGC

5、AAGCGAACCGGAATTGCCAGCTGGGGCGCCCTCTGGTAAGGTTGGGAAGCCCTGCAAAGTAAACTGGATGGCTTTCTTGCCGCCAAGGATCTGATGGCGCAGGGGATCAAGATCTGATCAAGAGACAGGATGAGGATCGTTTCGCATGATTGAACAAGATGGATTGCACGCAGGTTCTCCGGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCACAACAGACAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGA

6、CCTGTCCGGTGCCCTGAATGAACTGCAGGACGAGGCAGCGCGGCTATCGTGGCTGGCCACGACGGGCGTTCCTTGCGCAGCTGTGCTCGACGTTGTCACTGAAGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGGATCTCCTGTCATCCCACCTTGCTCCTGCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGCGGCTGCATACGCTTGATCCGGCTACCTGCCCATTCGACCACCAAGCGAAACATCGCATCGAGCGAGCACGTACTCGGATGGAAGCCGGTCT

7、TGTCGATCAGGATGATCTGGACGAAGAGCATCAGGGGCTCGCGCCAGCCGAACTGTTCGCCAGGCTCAAGGCGCGCATGCCCGACGGCGAGGATCTCGTCGTGACCCATGGCGATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCCGCTTTTCTGGATTCATCGACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGACATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGACCGCTTCCTCGTGCTTTACGGTATCGCCGCTCCCGATTCGCA

8、GCGCATCGCCTTCTATCGCCTTCTTGACGAGTTCTTCTGAATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGT

9、ATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCT

10、GTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCA

11、CTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTA

12、ACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTT

13、CCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGAT

14、ACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGLacZa fragment: bases 1-548M13 reverse priming site: bases 205-221Multiple cloning site: bases 234-358T7 promoter/priming site: bases 364-381M13 Forward (-20) priming site: bases 389-404f1 origin: bases 546-983Kanamycin resistance ORF: bases

15、1317-2111Ampicillin resistance ORF: bases 2129-2989pUC origin: bases 3134-3807pEASY-Blunt Restriction MapEnzyme# of cutspositionsAcc65I (1)ggtacc246AceIII (3)cagctc949, 2630, 3870AciI (47)ccgc88, 191, 329, 561, 575, 1014, 1159, 1515, 1579, 1680AclI (2)aacgtt2319, 2692AcuI (4)ctgaag3283, 1576, 2008,

16、2235AflII (1)cttaag297AflIII (2)acrygt1021, 3810AhdI (1)gacnnnnngtc2922AloI (1)gaacnnnnnntcc835AluI (22)agct58, 122, 217, 239, 251, 477, 691, 948, 1069, 1122AlwI (16)ggatc258, 1700, 2292, 3072, 3169, 257, 1263, 1280, 1310, 1621AlwNI (2)cagnnnctg1073, 3399ApaBI (1)gcannnnntgc1002ApaI (1)gggccc356ApaL

17、I (2)gtgcac2250, 3496ApoI (3)raatty964, 975, 986AseI (3)attaat52, 111, 2746AvaI (1)cycgrg338AvaII (3)ggwcc1966, 2557, 2779BamHI (1)ggatcc258BanI (7)ggyrcc137, 246, 740, 1202, 1450, 1485, 2969BanII (4)grgcyc252, 356, 706, 1812BbeI (2)ggcgcc1202, 1450BbvI (18)gcagc1002, 1410, 1594, 1687, 2500, 3194, 3

18、400, 57, 457, 530BccI (11)ccatc771, 1243, 1269, 1334, 1762, 2470, 2757, 2881, 319, 789BceAI (6)acggc397, 1424, 3324, 270, 737, 1860BcefI (6)acggc397, 1424, 3324, 270, 737, 1860BcgI (1)cgannnnnntgc2401BciVI (2)gtatcc1659, 3601BclI (1)tgatca1292BfaI (7)ctag263, 349, 626, 1150, 2727, 3062, 3315BfrBI (1

19、)atgcat345BglI (2)gccnnnnnggc539, 2803BglII (1)agatct1287Bme1580I (5)gkgcmc356, 1393, 1486, 2250, 3496BmrI (5)actggg420, 1075, 1156, 1390, 2872BpmI (1)ctggag2832BpuEI (5)cttgag1842, 3442, 2297, 3165, 3704BsaAI (3)yacgtr779, 1022, 1753BsaBI (3)gatnnnnatc319, 1286, 1310BsaHI (3)grcgyc1202, 1450, 2381B

20、saI (1)ggtctc2850BsaJI (7)ccnngg142, 430, 1056, 1260, 1614, 1883, 3650BsaWI (5)wccggw1184, 1482, 2626, 3457, 3604BsaXI (2)acnnnnnctcc833, 28BscAI (11)gcatc1418, 1673, 1889, 2230, 2670, 3722, 346, 1740, 1804, 2079BseMII (4)ctcag2960, 3126, 3535, 2420BseYI (3)cccagc1197, 1955, 3506BsiEI (8)cgrycg272,

21、332, 508, 1357, 2401, 2550, 3473, 3897BsiHKAI (6)gwgcwc252, 1560, 1750, 2250, 2335, 3496BslI (9)ccnnnnnnngg39, 561, 887, 1431, 1975, 3331, 3610, 3776, 3794BsmAI (2)gtctc1298, 2850BsmFI (1)gggac1587Bsp1286I (11)gdgchc252, 356, 706, 1393, 1486, 1560, 1750, 1812, 2250, 2335Bsp24I (1)gacnnnnnntgg3130Bsp

22、CNI (4)ctcag2960, 3126, 3535, 2420BspHI (1)tcatga3090BspMI (3)acctgc1118, 1347, 1710BsrBI (5)ccgctc191, 335, 635, 2062, 3879BsrDI (3)gcaatg2863, 1677, 2681BsrFI (4)rccggy676, 1770, 1951, 2837BsrI (20)actgg277, 1032, 2446, 3394, 3407, 81, 326, 393, 419, 864BssHII (1)gcgcgc1848BssSI (3)cacgag2043, 225

23、3, 3637BstAPI (1)gcannnnntgc1002BstF5I (10)ggatg463, 1632, 1059, 1242, 1304, 1761, 1786, 2469, 2756, 2937BstKTI (25)gatc257, 507, 1263, 1280, 1286, 1291, 1310, 1621, 1699, 1780BstNI (6)ccwgg142, 430, 1836, 3649, 3662, 3783BstUI (12)cgcg33, 550, 574, 594, 970, 1514, 1815, 1847, 2360, 2853BstXI (2)cca

24、nnnnnntgg284, 326BstYI (11)rgatcy258, 1264, 1287, 1622, 1868, 2275, 2292, 3060, 3072, 3158BtgI (1)ccrygg1883BthCI (34)gcngc57, 273, 330, 457, 530, 562, 576, 598, 1002, 1255BtsI (4)gcagtg2524, 94, 1129, 2496Cac8I (24)gcnngc61, 90, 343, 476, 500, 619, 633, 676, 1101, 1125CdiI (7)catcg1888, 2669, 790,

25、1736, 1939, 2080, 2265ChaI (25)gatc257, 507, 1263, 1280, 1286, 1291, 1310, 1621, 1699, 1780CjeI (7)ccannnnnngt3228, 3783, 243, 482, 3129, 3229, 3307CjePI (4)ccannnnnnntc3277, 2538, 3010, 3130Csp6I (3)gtac245, 1754, 2438CviAII (14)catg226, 342, 1139, 1320, 1665, 1851, 1882, 1908, 2474, 2510CviJI (78)

26、rgcy41, 58, 122, 144, 165, 217, 239, 251, 271, 331DdeI (4)ctnag2420, 2960, 3126, 3535DpnI (25)gatc257, 507, 1263, 1280, 1286, 1291, 1310, 1621, 1699, 1780DraI (3)tttaaa2342, 3034, 3053DraIII (1)cacnnngtg782DrdI (3)gacnnnnnngtc828, 1478, 3708EaeI (9)yggccr42, 272, 332, 396, 1357, 1531, 1922, 1949, 25

27、29EagI (3)cggccg272, 332, 1357EarI (4)ctcttc494, 1800, 2010, 2128EciI (4)ggcgga1964, 2764, 3592, 3738Eco57MI (5)ctgrag3283, 1576, 2008, 2235, 2832EcoHI (6)ccsgg1056, 1453, 1613, 2384, 2735, 3431EcoICRI (1)gagctc252EcoO109I (1)rggnccy356EcoRV (1)gatatc315EsaBC3I (9)tcga337, 744, 1562, 1718, 1742, 177

28、8, 1940, 2266, 3710FalI (1)aagnnnnnctt1773FatI (14)catg226, 342, 1139, 1320, 1665, 1851, 1882, 1908, 2474, 2510FauI (6)cccgc89, 579, 1580, 32, 500, 633FmuI (8)ggncc355, 498, 786, 1966, 2557, 2779, 2796, 2875Fnu4HI (34)gcngc57, 273, 330, 457, 530, 562, 576, 598, 1002, 1255FokI (10)ggatg463, 1632, 105

29、9, 1242, 1304, 1761, 1786, 2469, 2756, 2937FspI (3)tgcgca528, 1551, 2697GdiII (11)cggccr42, 272, 332, 396, 1357, 1922, 1949, 272, 332, 1357HaeI (4)wggccw1531, 3334, 3786, 3797HaeII (6)rgcgcy7, 622, 630, 1202, 1450, 3566HaeIII (19)ggcc41, 271, 331, 355, 395, 497, 786, 928, 1356, 1530HaeIV (2)gaynnnnn

30、rtc1628, 2922HgaI (4)gacgc3130, 3708, 549, 2380HhaI (30)gcgc6, 34, 99, 527, 551, 564, 573, 595, 621, 629Hin4I (8)gaynnnnnvtc257, 346, 1628, 1658, 2848, 2922, 1628, 2922HindIII (1)aagctt240HinfI (10)gantc46, 376, 828, 850, 1935, 2069, 2922, 3439, 3835, 3910HinP1I (30)gcgc6, 34, 99, 527, 551, 564, 573

31、, 595, 621, 629HpaII (21)ccgg163, 675, 1056, 1183, 1354, 1431, 1453, 1481, 1612, 1702HphI (6)ggtga2202, 2443, 768, 2217, 2839, 3066Hpy188I (15)tcnga255, 721, 1266, 1289, 1415, 1759, 2112, 2421, 2541, 2552Hpy188III (21)tcnnga77, 350, 415, 1084, 1284, 1295, 1468, 1784, 1793, 1876Hpy8I (7)gtnnac776, 83

32、1, 848, 1236, 2250, 3008, 3496Hpy99I (9)cgwcg399, 412, 1536, 1564, 1858, 1873, 2652, 2915, 3709HpyAV (10)ccttc1584, 2562, 3270, 3580, 514, 654, 2085, 2095, 2187, 2799HpyCH4I (14)catg226, 342, 1139, 1320, 1665, 1851, 1882, 1908, 2474, 2510HpyCH4III (11)acngt522, 804, 1045, 1828, 1944, 2052, 2479, 299

33、4, 3307, 3777HpyCH4IV (11)acgt410, 668, 778, 821, 833, 1021, 1565, 1752, 2318, 2691HpyCH4V (17)tgca55, 309, 344, 455, 1126, 1228, 1340, 1499, 1674, 1688HpyF10VI (25)gcnnnnnnngc15, 99, 143, 290, 339, 363, 509, 539, 571, 615KasI (2)ggcgcc1202, 1450KpnI (1)ggtacc246LpnI (6)rgcgcy7, 622, 630, 1202, 1450

34、, 3566M.AacDam (25)gatc257, 507, 1263, 1280, 1286, 1291, 1310, 1621, 1699, 1780M.AclI (2)aacgtt2319, 2692M.AflIII (2)acrygt1021, 3810M.AlaK2I (25)gatc257, 507, 1263, 1280, 1286, 1291, 1310, 1621, 1699, 1780M.AloI (1)gaacnnnnnntcc835M.AluI (22)agct58, 122, 217, 239, 251, 477, 691, 948, 1069, 1122M.Al

35、w26I (2)gtctc1298, 2850M.AlwI (16)ggatc258, 1700, 2292, 3072, 3169, 257, 1263, 1280, 1310, 1621M.ApaI (1)gggccc356M.AquI (1)cycgrg338M.AvaIX (4)rccggy676, 1770, 1951, 2837M.AvaV (25)gatc257, 507, 1263, 1280, 1286, 1291, 1310, 1621, 1699, 1780M.AvaVI (25)gatc257, 507, 1263, 1280, 1286, 1291, 1310, 16

36、21, 1699, 1780M.AvaVII (19)ggcc41, 271, 331, 355, 395, 497, 786, 928, 1356, 1530M.BabI (10)gantc46, 376, 828, 850, 1935, 2069, 2922, 3439, 3835, 3910M.BanII (4)grgcyc252, 356, 706, 1812M.BbvI (18)gcagc1002, 1410, 1594, 1687, 2500, 3194, 3400, 57, 457, 530M.BbvSI (18)gcwgc57, 457, 530, 598, 1002, 141

37、0, 1511, 1553, 1594, 1687M.BcgI (1)cgannnnnntgc2401M.BclI (1)tgatca1292M.BcnIA (6)ccsgg1056, 1453, 1613, 2384, 2735, 3431M.BcnIB (6)ccsgg1056, 1453, 1613, 2384, 2735, 3431M.BepI (12)cgcg33, 550, 574, 594, 970, 1514, 1815, 1847, 2360, 2853M.BfiIA (5)actggg420, 1075, 1156, 1390, 2872M.BfiIB (5)actggg4

38、20, 1075, 1156, 1390, 2872M.BflBF4I (18)gcsgc273, 330, 562, 576, 1255, 1358, 1421, 1516, 1681, 1923M.BglII (1)agatct1287M.BmeTI (1)tgatca1292M.BnaI (1)ggatcc258M.BsaAI (3)yacgtr779, 1022, 1753M.BspKT6I (25)gatc257, 507, 1263, 1280, 1286, 1291, 1310, 1621, 1699, 1780M.BspLU11IIIA (1)gggac1587M.BspLU1

39、1IIIC (1)gggac1587M.BspRI (19)ggcc41, 271, 331, 355, 395, 497, 786, 928, 1356, 1530M.BspST5I (11)gcatc1418, 1673, 1889, 2230, 2670, 3722, 346, 1740, 1804, 2079M.BstF5IA (10)ggatg463, 1632, 1059, 1242, 1304, 1761, 1786, 2469, 2756, 2937M.BstF5ID (10)ggatg463, 1632, 1059, 1242, 1304, 1761, 1786, 2469,

40、 2756, 2937M.BstI (1)ggatcc258M.BstNI (6)ccwgg142, 430, 1836, 3649, 3662, 3783M.BstVI (1)ctcgag338M.BstYI (11)rgatcy258, 1264, 1287, 1622, 1868, 2275, 2292, 3060, 3072, 3158M.BsuBI (2)ctgcag310, 1500M.BsuEII (12)cgcg33, 550, 574, 594, 970, 1514, 1815, 1847, 2360, 2853M.BsuFI (21)ccgg163, 675, 1056,

41、1183, 1354, 1431, 1453, 1481, 1612, 1702M.BsuRI (19)ggcc41, 271, 331, 355, 395, 497, 786, 928, 1356, 1530M.CboI (21)ccgg163, 675, 1056, 1183, 1354, 1431, 1453, 1481, 1612, 1702M.CcrMI (10)gantc46, 376, 828, 850, 1935, 2069, 2922, 3439, 3835, 3910M.Cfr10I (4)rccggy676, 1770, 1951, 2837M.Cfr13I (8)ggn

42、cc355, 498, 786, 1966, 2557, 2779, 2796, 2875M.Cfr6I (4)cagctg59, 478, 1195, 1555M.CfrBI (2)ccwwgg1260, 1883M.CfrI (9)yggccr42, 272, 332, 396, 1357, 1531, 1922, 1949, 2529M.CglASI (18)gcsgc273, 330, 562, 576, 1255, 1358, 1421, 1516, 1681, 1923M.CviAI (25)gatc257, 507, 1263, 1280, 1286, 1291, 1310, 1

43、621, 1699, 1780M.CviAII (14)catg226, 342, 1139, 1320, 1665, 1851, 1882, 1908, 2474, 2510M.CviAIV (260)rgcb7, 23, 41, 58, 122, 144, 165, 217, 234, 239M.CviBI (10)gantc46, 376, 828, 850, 1935, 2069, 2922, 3439, 3835, 3910M.CviBIII (9)tcga337, 744, 1562, 1718, 1742, 1778, 1940, 2266, 3710M.CviJI (260)r

44、gcb7, 23, 41, 58, 122, 144, 165, 217, 234, 239M.CviPI (309)gc4, 15, 22, 32, 40, 54, 57, 61, 86, 90M.CviQI (3)gtac245, 1754, 2438M.CviQIII (9)tcga337, 744, 1562, 1718, 1742, 1778, 1940, 2266, 3710M.CviQVI (10)gantc46, 376, 828, 850, 1935, 2069, 2922, 3439, 3835, 3910M.CviQVII (14)catg226, 342, 1139,

45、1320, 1665, 1851, 1882, 1908, 2474, 2510M.CviRI (17)tgca55, 309, 344, 455, 1126, 1228, 1340, 1499, 1674, 1688M.CviRII (3)gtac245, 1754, 2438M.CviSI (17)tgca55, 309, 344, 455, 1126, 1228, 1340, 1499, 1674, 1688M.CviSII (14)catg226, 342, 1139, 1320, 1665, 1851, 1882, 1908, 2474, 2510M.CviSIII (9)tcga3

46、37, 744, 1562, 1718, 1742, 1778, 1940, 2266, 3710M.CviTI (78)rgcy41, 58, 122, 144, 165, 217, 239, 251, 271, 331M.DdeI (4)ctnag2420, 2960, 3126, 3535M.DpnIIA (25)gatc257, 507, 1263, 1280, 1286, 1291, 1310, 1621, 1699, 1780M.DpnIIB (25)gatc257, 507, 1263, 1280, 1286, 1291, 1310, 1621, 1699, 1780M.DraI

47、 (3)tttaaa2342, 3034, 3053M.DraIII (1)cacnnngtg782M.DsaV (12)ccngg142, 430, 1056, 1453, 1613, 1836, 2384, 2735, 3431, 3649M.EaeI (9)yggccr42, 272, 332, 396, 1357, 1531, 1922, 1949, 2529M.Ecl18kI (12)ccngg142, 430, 1056, 1453, 1613, 1836, 2384, 2735, 3431, 3649M.Eco1831I (6)ccsgg1056, 1453, 1613, 238

48、4, 2735, 3431M.Eco57IA (4)ctgaag3283, 1576, 2008, 2235M.Eco57IB (4)ctgaag3283, 1576, 2008, 2235M.Eco67Dam (25)gatc257, 507, 1263, 1280, 1286, 1291, 1310, 1621, 1699, 1780M.EcoHI (6)ccsgg1056, 1453, 1613, 2384, 2735, 3431M.EcoHK31I (9)yggccr42, 272, 332, 396, 1357, 1531, 1922, 1949, 2529M.EcoKDam (25

49、)gatc257, 507, 1263, 1280, 1286, 1291, 1310, 1621, 1699, 1780M.EcoKDcm (6)ccwgg142, 430, 1836, 3649, 3662, 3783M.EcoKI (1)aacnnnnnngtgc2259M.EcoO109I (1)rggnccy356M.EcoP15I (3)cagcag3193, 3402, 3286M.EcoP1Dam (25)gatc257, 507, 1263, 1280, 1286, 1291, 1310, 1621, 1699, 1780M.EcoPI (6)agacc890, 1772,

50、2849, 1470, 3000, 3135M.EcoR124II (1)gaannnnnnnrtcg1741M.EcoRII (6)ccwgg142, 430, 1836, 3649, 3662, 3783M.EcoRV (1)gatatc315M.EcoT1Dam (25)gatc257, 507, 1263, 1280, 1286, 1291, 1310, 1621, 1699, 1780M.EcoT2Dam (25)gatc257, 507, 1263, 1280, 1286, 1291, 1310, 1621, 1699, 1780M.EcoT4Dam (25)gatc257, 50

51、7, 1263, 1280, 1286, 1291, 1310, 1621, 1699, 1780M.EsaBC1I (22)agct58, 122, 217, 239, 251, 477, 691, 948, 1069, 1122M.EsaBC3I (9)tcga337, 744, 1562, 1718, 1742, 1778, 1940, 2266, 3710M.EsaBS1I (14)catg226, 342, 1139, 1320, 1665, 1851, 1882, 1908, 2474, 2510M.EsaDix3I (9)tcga337, 744, 1562, 1718, 174

52、2, 1778, 1940, 2266, 3710M.EsaDix4I (18)ttaa51, 110, 296, 445, 569, 840, 938, 955, 966, 978M.EsaDix5I (18)ttaa51, 110, 296, 445, 569, 840, 938, 955, 966, 978M.EsaLHCI (25)gatc257, 507, 1263, 1280, 1286, 1291, 1310, 1621, 1699, 1780M.FnuDII (12)cgcg33, 550, 574, 594, 970, 1514, 1815, 1847, 2360, 2853

53、M.FokI (10)ggatg463, 1632, 1059, 1242, 1304, 1761, 1786, 2469, 2756, 2937M.HaeIII (19)ggcc41, 271, 331, 355, 395, 497, 786, 928, 1356, 1530M.HaeIV (2)gaynnnnnrtc1628, 2922M.HapII (21)ccgg163, 675, 1056, 1183, 1354, 1431, 1453, 1481, 1612, 1702M.HgaIA (4)gacgc3130, 3708, 549, 2380M.HgaIB (4)gacgc3130

54、, 3708, 549, 2380M.HgiCI (7)ggyrcc137, 246, 740, 1202, 1450, 1485, 2969M.HhaI (30)gcgc6, 34, 99, 527, 551, 564, 573, 595, 621, 629M.HhaII (10)gantc46, 376, 828, 850, 1935, 2069, 2922, 3439, 3835, 3910M.HindDam (25)gatc257, 507, 1263, 1280, 1286, 1291, 1310, 1621, 1699, 1780M.HindI (99)cac94, 114, 176, 186, 278, 372, 414, 581, 586, 601M.HindIII (1)aagctt240M.HinfI (10)gantc46, 376, 828, 850, 1935, 2069, 2922, 3439, 3835, 3910M.HinHP1Dam (25)gatc257, 507, 1263, 1280, 1286, 1291, 1310, 1621, 1699, 1780M.HinP1I (30)gcgc6, 34, 99, 527, 551,