濕熱消毒滅菌法主講老師：徐單單WetheatdisinfectionandsterilizationmethodGuangdongFoodandDrugVocationalCollege廣東食品藥品職業學院濕熱消毒滅菌法01Wetheatdisinfectionandsterilizationmethod01濕熱消毒滅菌法Wetheatdisinfectionandsterilizationmethod濕熱消毒滅菌法Wetheatdisinfectionandsterilizationmethod煮沸消毒法Boilingdisinfectionmethod1流通蒸汽消毒法Circulatingsteamdisinfectionmethod2間歇蒸汽滅菌法Fractionalsterilization3巴氏消毒法Pasteurizationmethod4高壓蒸汽滅菌法Sterilizationbypressuredsteam5煮沸消毒法Boilingdisinfectionmethod濕熱消毒滅菌法Wetheatdisinfectionandsterilizationmethod100℃，5-10min消毒Disinfectat100℃for5-10minutes適用于飲水、餐具、刀、剪、小容量口服液和注射劑Suitablefordrinkingwater,tableware,knives,scissors,smallvolumeoralliquids,andinjections流通蒸汽消毒法Circulatingsteamdisinfectionmethod濕熱消毒滅菌法Wetheatdisinfectionandsterilizationmethod

100℃，5-10min消毒Disinfectat100℃for5-10minutes餐具、口服液、管道、小容量口服液和注射劑Tableware,oralliquids,tubing,smallvolumeoralliquids,andinjections間歇蒸汽滅菌法Intermittentsteamsterilizationmethod濕熱消毒滅菌法Wetheatdisinfectionandsterilizationmethod100℃連續三天每天蒸汽一次可滅菌Steamingonceadayforthreeconsecutivedaysat100℃cansterilize適用于管道或不耐高溫的培養基、藥品、營養液滅菌Suitableforsterilizationofpipelinesorculturemedia,drugs,andnutrientsolutionsthatarenotresistanttohightemperatures濕熱消毒滅菌法Wetheatdisinfectionandsterilizationmethod63℃

30min

或

72℃15s消毒適用范圍：牛奶、奶酪、果汁、酒類、糖漿、果脯、醬菜等食品消毒、注射用水儲藏優點不破壞營養成分不影響風味缺點只消毒，不滅菌巴氏消毒法(低溫消毒法)圖1.巴氏消毒法的適用范圍

Figure1.ScopeofApplicationofPasteurizationMethod高壓蒸汽滅菌法High-pressuresteamsterilizationmethod濕熱消毒滅菌法Wetheatdisinfectionandsterilizationmethod方法1：121.3℃，103.42KPa(1.05kg/cm2,15Ib/in2)15-30minMethod1:121.3℃,103.42KPa(1.05kg/cm2,15Ib/in2)15-30min方法2：115℃，68.95KPa(0.703kg/cm2,10Ib/in2)30minMethod2:At115℃,68.95KPa(0.703kg/cm2,10Ib/in2)for30min濕熱消毒滅菌法Wetheatdisinfectionandsterilizationmethod適用范圍：耐高溫不怕濕的物品，微生物實驗材料、藥品生產、醫療用品

Scopeofapplication:Itemsthatareresistanttohightemperaturesandnot

afraidofmoisture，microbialexperimentalmaterials,drugproduction,

medicalsupplies優點：快速滅菌，濕熱滅菌法中最好的方法Advantages:Rapidsterilization,thebestmethodamongmoistheatsterilizationmethods缺點：滅菌后物品潮濕Disadvantage:Itemsbecomedampaftersterilization

①

高壓蒸汽滅菌法的具體適用范圍①

Thespecificscopeofapplicationofhigh-pressuresteam

sterilizationmethod微生物實驗，普通培養基、生理鹽水、玻璃器皿、金屬、陶瓷制品Microbialexperiment,ordinaryculturemedium,physiologicalsaline,glassware,metal,ceramicproducts藥品生產，化學原料、植物原料、輔料、注射劑（如輸液）、膠塞、容器、

工作服、制、儲水設備、管道Drugproduction,chemicalrawmaterials,plantrawmaterials,excipients,injections(suchasinfusion),stoppers,containers,workclothes,waterstorageequipment,pipelines醫療用品，無菌器械、敷料、手術用具、手術衣等Medicalsupplies,sterileinstruments,dressings,surgicaltools,surgicalgowns,etc圖2.高壓蒸汽滅菌法的適用范圍

Figure2.Scopeofapplicationofhigh-pressuresteamsterilizationmethod圖3.高壓蒸汽滅菌法的適用范圍

Figure3.Scopeofapplicationofhigh-pressuresteamsterilizationmethod圖4.高壓蒸汽滅菌法的適用范圍

Figure4.Scopeofapplicationofhigh-pressuresteamsterilizationmethod圖5.手提式滅菌鍋

Figure5.Portablesterilizationpot②高壓蒸汽滅菌器類型:手提式、立式、臥式圖6.立式滅菌鍋

Figure6.Verticalsterilizationpot圖7.臥式滅菌鍋

Figure7.Horizontalsterilizationpot③高壓蒸汽滅菌注意事項③Precautionsforhigh-pressuresteamsterilization

定期檢查壓力表是否靈敏，壓力與溫度是否對應；Regularlycheckwhetherthepressuregaugeissensitiveandwhetherthepressurecorrespondstothetemperature滅菌初期先排凈鍋內冷空氣;Duringtheinitialstageofsterilization,thecoldairinsidethepotshouldbepurgedfirst滅菌時間計算：達到規定壓力和溫度開始計；Sterilizationtimecalculation:startcountingwhenthespecifiedpressureandtemperaturearereached

鍋內水分要充足；Thewaterinthepotshouldbesufficient滅菌物品裝量不宜太滿；Theamountofsterilizeditemsshouldnotbetoofull滅菌結束需緩慢減壓；Slowdecompressionisrequiredaftersterilizationiscompleted滅菌結束，趁熱取出物品。Sterilizationiscomplete,takeouttheitemswhiletheyarestillhot小結：以上就是我們這次課的主要學習內容，主要講述了濕熱消毒滅菌法，重點講述高壓蒸汽滅菌法、適用范圍、滅菌鍋類型。Summary:Theaboveisthemaincontentofourlesson,which