Lab6Slides:1.Bloodsmear（NO.8）2.Smallartery、smallveinandcapillary(NO.18）3.Medium–sizedarteryandvein（NO.19）4.Largeartery(elasticartery)（NO.20）Homework:1.bloodcell2.medium-sizedarteryandvein(crosssection)ObjectiveMasterandrecognizetheformedelementsofblood.Masterandrecognizethemedium-sizedarteries,smallarteriesandcapillaries.Befamiliarwithlargearteries,medium-sizedandsmallveins.Befamiliarwiththeelectronmicrographsofcontinuous,fenesratedandsinusoidalcapillaries.

SlideNo:8Material:humanbloodMethod:Giemsastaininghemogramindex:erythrocyte：

3.5~5.5×1012/Lhemoglobin:100~150g/LLeukocyte:4~10×109/LPlatelets:100~300×109/LLowpower:1.erythrocyte2.leukocyte12Erythrocyte

biconcavediscsinshapenonucleusandorganellafilledwithhemoglobin7.5-8.5umindiameterReticulocytesAreslightlyimmatureredbloodcells.Adult：0.5%-1.5%Newborn：3%-6%Reticulocyte（網織紅細胞）Method:brilliantcresylblue(煌焦油藍）stainingNeutrophilround,10-12umindiameterrob-likedorpolymorphousnucleus,2-5lobesfineneutrophilicgranules-pinkcytoplasm4~5葉分葉核的細胞百分率增多→核右移(核分葉越多→衰老)rodnucleus：5~10%在某些疾病情況下，桿狀核的細胞百分率增多

核左移NeutrophilEMEosinophlis

round,10-15umindiameter2lobesnucleusroughbrightredgranules---acidophilicgranulesEMBasophilless,round10-12um“S”shapedorirregularnucleuscoarsedark-purplegranules---basophilicgranulesEMLymphocyteNucleus：roundnucleuswithindentation,staineddeeppurple.Cytoplasm:brightblueincolorEMMonocytelargeround,14-20umNucleus:askidney,horse-shoeovoidinshapecytoplasm:gray-blueincolorEM（LM）Bloodplatelet（EM）Bloodplatelet特殊顆粒MonocyteLymphocytePlateletsSmallarteryandvein,capillary(NO.18)

Material:smallarteryandveinMethod:H.EstainingTunicaIntimae*InternalelasticmembraneTunicamediaTunicaadventia*externalelasticmembraneSmallArterySmallveinhighpowercapillary

(H.E)Lowpowercapillary

(H.E)

capillarySmallArteryandvein

(H.E)2112

較大的小動脈，管壁三層結構較完整，內彈性膜明顯。1.小動脈2.內彈性膜3.小靜脈：管壁薄，管腔不規則。312Medium-sizedarteryandvein(NO.19)Material:medium-sizedarteryandveinMethod:H.EstainingTunicaIntimae*InternalelasticmembraneTunicamedia(10-40layers)Tunicaadventia*externalelasticmembraneThewallofMedium-sizedarteryAdventitiamediaIntimaeInt.elasticmemExt.elasticmemInternalelasticmembraneExternalelasticmembraneElasticfiberMedium-sizedartery123Payattentiontothreelayersoffilmthicknessratio.Medium-sizedarteryMedium-sizedveinMedium-sizedarteryPayattentiontothreelayersoffilmthicknessratio.123Mediumsizedvein1.endothelium2.Subendotheliallayer3.Smoothmuscle123Largeartery(elasticartery,NO.19)Material:largearteryMethod:H.EstainingTunicaIntimaeTunicamediaTunicaadventiaHighpower：1.endothelium2.Subendotheliallayer3.Elasticlaminas4.Smoothmuscle1234Highpower：Tunicamedia1.Elasticlaminas2.Smoothmuscle*Noticetheelasticlaminasinthetunicamedia(40-70layers).12mediaintimaeadventitiaLargearteryLargeveinadventitiamediaintimaeSmoothmusclebundlesTunicaadventitiaEM1.pericyte2.Continuedcapillary

3.FenestratedcapillaryThecapillaryhole4.sinusoid

Theend1、硬膜（duramater）硬膜外腔（epiduralspace）

硬膜下隙