1、各 種 激 發 光 和 吸 收 光 波 長現在發現一個更全的有關激發光和發射光的波長圖譜。這對CONFOCAL去做多重染色時是必不可少的參考資料。 全部文件Excitation and Emission Wavelengths of FluorophoresTavis FluoroTable: Common fluorophores (from )FluorophoreAbsorptionEmission Other info1,5 IAEDANS3364901,8-ANS3724804-Methylumbelliferone3855025-carboxy-2,7-dichlorofluore

2、scein5045295-Carboxyfluorescein (5-FAM)4925185-Carboxynapthofluorescein (pH 10)512/598563/668 Ratio Dye, pH5-Carboxytetramethylrhodamine542568(5-TAMRA)5-FAM (5-Carboxyfluorescein)4925185-HAT (Hydroxy Tryptamine)370-415520-5405-Hydroxy Tryptamine (HAT)370-415520-5405-ROX (carboxy-X-rhodamine)57860456

3、75915-TAMRA548552(5-Carboxytetramethylrhodamine)5425686-Carboxyrhodamine 6G5185436-CR 6G5185436-JOE5205487-Amino-4-methylcoumarin3514307-Aminoactinomycin D (7-AAD)5466477-Hydroxy-4-methylcoumarin360449, 4559-Amino-6-chloro-2-methoxyacridine412,430471,474ABQ344445Acid Fuchsin540630ACMA412,430471,474(

4、9-Amino-6-chloro-2-methoxyacridine)Acridine Orange + DNA502526Acridine Orange + RNA460650Acridine Orange, both DNA & RNA440-480520-650Acridine Red455-600560-680Acridine Yellow470550Acriflavin436520Acriflavin Feulgen SITSA355-425460Aequorin (Photoprotein)466PhotoproteinAFPs - AutoFluorescent Protein-

5、(Quantum Biotechnologies) see sgGFP,sgBFPAlexa Fluor 350346442342441Alexa Fluor 430431540Anilin Blue600Alexa Fluor 488495, 492519, 520Alexa Fluor 532531, 532553, 554Alexa Fluor 546556, 557572, 573Alexa Fluor 568577, 578603Alexa Fluor 594590, 594617, 618Alexa Fluor 633632650Alexa Fluor 647647666Alexa

6、 Fluor 660668698Alexa Fluor 680679702Alizarin Complexon530-560,580,580624-645Alizarin Red530-560580Allophycocyanin (APC)630, 645655, 660AMC, AMCA-S345445AMCA (Aminomethylcoumarin)345425347444AMCA-X353442Aminoactinomycin D555655Aminocoumarin346442350445Aminomethylcoumarin (AMCA)345425347444Blue Fluor

7、escentAnthrocyl stearate360-381446APC (Allophycocyanin)630, 645655, 660APC-Cy7625-650755APTRA-BTC = Ratio Dye, Zn2+466/380520/530 Ratio Dye, Zn2+APTS424505Astrazon Brilliant Red 4G500585Astrazon Orange R470540Astrazon Red 6B520595Astrazon Yellow 7 GLL450480Atabrine436490ATTO-TAG CBQCA465560ATTO-TAG

8、FQ486591Auramine460550Aurophosphine G450580Aurophosphine450-490515BAO 9(Bisaminophenyloxadiazole)365395BCECF (high pH)492, 503520,528BCECF (low pH)482520Berberine Sulphate430550Beta Lactamase409447, 520BFP blue shifted GFP (Y66H)381,382, 445,blue shifted GFPBlue Fluorescent Protein383447, 448 (Y66H)

9、ProteinBFP/GFP FRETBimane398490Bisbenzamide360461Bisbenzimide (Hoechst)360461bis-BTC = Ratio Dye, Zn2+455/405529/505 Ratio Dye, Zn2+Blancophor FFG390470Blancophor SV370435BOBO-1462481BOBO-3570602Bodipy 492/515490515Bodipy 493/503533549Bodipy 500/510509515Bodipy 505/515502510Bodipy 530/550528547Bodip

10、y 542/563543563Bodipy 558/568558569Bodipy 564/570564570Bodipy 576/589579590Bodipy 581/591584592Bodipy 630/650-X625642Bodipy 650/665-X647665Bodipy 665/676605676Bodipy Fl504,505511, 513Bodipy FL ATP505514Bodipy Fl-Ceramide505511Bodipy R6G SE528547Bodipy TMR542574Bodipy TMR-X conjugate544573Bodipy TMR-

11、X, SE544570Bodipy TR589617Bodipy TR ATP591620Bodipy TR-X SE588616BO-PRO-1462481BO-PRO-3544570Brilliant Sulphoflavin FF430520BTC - Ratio Dye Ca2+464/401533/529 Ratio Dye Ca2+BTC-5N - atio Dye, Zn2+459/417517/532 Ratio Dye, Zn2+Calcein494517Calcein Blue373440Calcium Crimson588, 589611,615Calcium Green

12、501, 506531Calcium Green-1 Ca2+ Dye506531Ca2+ DyeCalcium Green-2 Ca2+506/503536Ca2+Calcium Green-5N Ca2+506532Ca2+Calcium Green-C18 Ca2+509530Ca2+Calcium Orange549575576Calcofluor White385,395, 437,405440, 445Carboxy-X-rhodamine (5-ROX)576601Cascade Blue377420398423399Cascade Yellow399550400552Catec

13、holamine410470CCF2 (GeneBlazer)CFDA494520CFP - Cyan Fluorescent Protein430,433, 474,436, (453)475,476,(501)CFP/YFP FRETChlorophyll480650Chromomycin A436-460470Chromomycin A445575CL-NERF (Ratio Dye, pH)504/514540CMFDA494520Cyan FluorescentProteinRatio Dye, pHCoelenterazineCa2+Dye, (429)465Ca2+Dye,bio

14、luminescenceCoelenterazine cp (Ca2+ Dye,)(430)442bioluminescence,native moleculeCa2+Dye,Coelenterazine f(437)473bioluminescenceCa2+Dye,Coelenterazine fcpCoelenterazine h(437)452464bioluminescenceCa2+Dye,bioluminescenceCa2+Dye,Coelenterazine hcp(433)444bioluminescenceCa2+Dye,Coelenterazine ipCoelente

15、razine n(431)441467bioluminescenceCa2+Dye,bioluminescenceCa2+Dye,Coelenterazine OCoumarin PhalloidinC-phycocyanineCPM Methylcoumarin460387384575470469bioluminescenceMethylcoumarinCTC400-450602CTC FormazanCy24895068554568581598Cy35145665525705548649666675695Cy5649666670Cy7710, 743767, 805Cyan GFP433

16、(453) 475(501)cyclic AMP Fluorosensor (FiCRhR)500517CyQuant Cell Proliferation Assay480520Dabcyl453Dansyl340578Dansyl Amine337517Dansyl Cadaverine335518Dansyl Chloride372518Dansyl DHPE336517CellProliferationAssayDansyl fluoride356noneDAPI359461Dapoxyl403580Dapoxyl 2374574Dapoxyl 3373574DCFDA504529DC

17、FH(Dichlorodihydrofluorescein505535Diacetate)DDAO463607DHR (Dihydorhodamine 123)505534Di-4-ANEPPS496705Di-8-ANEPPS (non-ratio)488605498713DiA (4-Di-16-ASP)456591DichlorodihydrofluoresceinDiacetate 505535(DCFH)DiD - Lipophilic Tracer644665DiD (DiIC18(5)644665DIDS341415Dihydorhodamine 123 (DHR)505535D

18、iI (DiIC18(3)549, 551565Dinitrophenol349LipophilicTracerDiO (DiOC18(3)DiRDiR (DiIC18)DM-NERF (high pH)DNPDopamineDsRedDTAFDY-630-NHSDY-635-NHSEBFPECFPEGFP484, 487748750497/510349340558494621634383436488, 498501, 502780779540490-520583520660664447474507, 516LipophilicTracerRatio Dye, pHRed fluorescen

19、tproteinHemicyanelabelforproteins and DNAHemicyanelabelforproteins and DNAEnhancedFluorescentProteinEnhancedFluorescentProteinEnhancedBlue506FluorescentELF 97345530Eosin524545Erythrosin529, 532554,555Erythrosin ITC529555Ethidium Bromide510, 523595, 605Ethidium homodimer-1 (EthD-1)528617Euchrysin4305

20、40EukoLightEuropium (III) chlorideEYFP513, 520527, 532 EnhancedFluorescentProteinFast Blue360440FDA494520Feulgen (Pararosaniline)570625FIF (Formaldehyd Induced Fluorescence)405433FITC490, 494520, 525FITC Antibody493517Flazo Orange375-530612Fluo-3480506, 520, 527ProteinYellowFluo-4494516Fluorescein (

21、FITC)490, 494520, 525Fluorescein Diacetate494520Fluoro-Emerald495524Fluoro-Gold (Hydroxystilbamidine)361536Fluor-Ruby555582FluorX494520FM 1-43479598FM 4-46515640Fura Red(high pH)572657Fura RedFluo-3Fura-2, high calcium335505Excitation ratiodyeFura-2, low calcium363512Excitation ratiodyeFura-2/BCECFG

22、enacryl Brilliant Red B520590Genacryl Brilliant Yellow 10GF430485Genacryl Pink 3G470583Genacryl Yellow 5GF430475GeneBlazer (CCF2)GFP (S65T)498516GFP red shifted (rsGFP)498516GFP wild type,non-UVexcitation 475509(wtGFP)GFP wild type, UV excitation(wtGFP)395509GFPuv385508Gloxalic Acid405460Granular Blue355425Haematoporphyrin530-560580Hoechst 33258345487Hoechst 33342347Filter sets installed on the Axiovert 200M:1) BrightfieldDIC:filter cube