1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemENicotinamideCat. No.: HY-B0150CAS No.: 98-92-0Synonyms: Niacinamide; Nicotinic acid amide; Vitamin B3分式: CHNO分量: 122.12作靶點: Endogenous Metabolite; Sirtuin作通路: Metabolic Enzyme/Protease; Cell Cycle/DNA Damage;Epigenetics儲存式: 4C,

2、protect from light* In solvent : -80C, 6 months; -20C, 1 month (protect fromlight)溶解性數據體外實驗 DMSO : 100 mg/mL (818.87 mM)H2O : 50 mg/mL (409.43 mM)* means soluble, but saturation unknown.Mass Solvent1 mg 5 mg 10 mg Concentration制備儲備液1 mM 8.1887 mL 40.9433 mL 81.8867 mL5 mM 1.6377 mL 8.1887 mL 16.3773

3、 mL10 mM 0.8189 mL 4.0943 mL 8.1887 mL請根據產品在不同溶劑中的溶解度，選擇合適的溶劑配制儲備液，并請注意儲備液的保存式和期限。體內實驗請根據您的實驗動物和給藥式選擇適當的溶解案，配制前請先配制澄清的儲備液，再依次添加助溶劑(為保證實驗結果的可靠性，體內實驗的作液，建議您現現配，當天使；澄清的儲備液可以根據儲存條件，適當保存；以下溶劑前的百分 指該溶劑在您配制終溶液中的體積占)：1. 請依序添加每種溶劑： 10% DMSO 40% PEG300 5% Tween-80 45% salineSolubility: 2.5 mg/mL (20.47 mM); C

4、lear solution2. 請依序添加每種溶劑： 10% DMSO 90% (20% SBE-CD in saline)1/3 Master of Small Molecules 您邊的抑制劑師www.MedChemESolubility: 2.5 mg/mL (20.47 mM); Clear solution3. 請依序添加每種溶劑： 10% DMSO 90% corn oilSolubility: 2.5 mg/mL (20.47 mM); Clear solutionBIOLOGICAL ACTIVITY物活性 Nicotinamide種B3維素，通過促進NAD +氧化還原穩態和提

5、供NAD +作為催化氧化還原反應的類酶的底物，在細胞理學中發揮重 作。 煙酰胺是SIRT1 的抑制劑。IC50 & Target PARP-1 Human Endogenous Metabolite體外研究 Pretreatment with the poly (ADP-ribose) polymerase (PARP) inhibitor nicotinamide is able to prevent HCN2cell death. When nicotinamide is added prior to t-BuOOH, it is able to prevent neuronal cell

6、 death and inhibitapoptosis. Nicotinamide-pretreated neurons have higher expression levels of inhibitors of apoptosis (IAP)genes 1. Nicotinamide inhibits vasoconstriction by ET. Nicotinamide also alleviates oxidative stress, whichexacerbates PE and FGR 3.體內研究 Normal and streptozotocin-nicotinamide i

7、nduced adult male diabetic rats receive quercetin (10, 25 and 50mg/kg/bw) orally, and cause significant decrease in FBG and cardiac injury marker levels with increased ininsulin levels 2. Nicotinamide improves maternal hypertension, proteinuria, and glomerular endotheliosis inRUPP mice. Moreover, ni

8、cotinamide prolongs pregnancies, and improves survival and growth of the embryosin RUPP PE mice 3.PROTOCOLAnimal DM is induced via a single intraperitoneal (i.p) injection of nicotinamide (110 mg/kg/body weight) dissolved inAdministration 2 normal saline 15 min prior to streptozotocin (STZ) (55 mg/k

9、g/body weight) injection, which is dissolved in afreshly prepared 0.1mol/Lcitrate buffer (pH 4.5). These injections are given following an overnight fast.Control rats (n=6) are injected with the same amount of solvent. In order to prevent hypoglycemia in the first24 h following STZ injection, rats a

10、re allowed to have free access to water with 5% dextrose (D5W). Threedays after STZ-nicotinamide injection, rats with FBG levels greater than 7.0 mM are considered as diabetic.MCE has not independently confirmed the accuracy of these methods. They are for reference only.戶使本產品發表的科研獻 Theranostics. 201

11、9 Apr 13;9(9):2424-2438. Hum Reprod. 2019 Jun 13. pii: dez068. J Mol Med (Berl). 2019 Jun 14. Patent. US20180263995A1.See more customer validations on HYPERLINK / www.MedChemE2/3 Master of Small Molecules 您邊的抑制劑師www.MedChemEREFERENCES1. Bhansali SG, et al. Nicotinamide prevents apoptosis in human co

12、rtical neuronal cells. Toxicol Mech Methods. 2006;16(4):173-80.2. Roslan J, et al. Quercetin ameliorates oxidative stress, inflammation and apoptosis in the heart of streptozotocin-nicotinamide-inducedadult male diabetic rats. Biomed Pharmacother. 2016 Dec 24;86:570-5823. Fushima T, et al. Nicotinam

13、ide ameliorates a preeclampsia-like condition in mice with reduced uterine perfusion pressure. Am J PhysiolRenal Physiol. 2016 Dec 7:ajprenal.00501.20164. Suzuki E, et al. Protective effect of nicotinamide against poly(ADP-ribose) polymerase-1-mediated astrocyte death depends on itstransporter-mediated uptake. Life Sci. 2010 Apr 24;86(17-18):676-82.MceP