1、Chapter 7Scale-up of Aerobic Processes7.1. Introduction of Scale-up反應(yīng)器內(nèi)的濃度場、溫度場、速度場分布顆粒團聚、氣泡聚并顆粒聚團內(nèi)的菌體細胞細胞內(nèi)代謝網(wǎng)絡(luò)基因DNA、RNA網(wǎng)絡(luò)ReactorCell metabilismGene express 放大過程基于細胞群體效應(yīng)和傳遞特性的直接放大新思路 搖瓶實驗室發(fā)酵罐工業(yè)化生產(chǎn)中試生產(chǎn)面向：傳統(tǒng)的生物過程逐級放大方法Scale up(1) inherent propertyGene influecnce metabilism (2)EnvironmentalMass trasfor

2、mationHomogenious system7.2. Power Calculations 7.1. Single stirrer in anaerated medium 7.2. Mutile stirrer in anaerated medium 7.3. Calculation of KLa Step by step scale up Mathmatical method Scale up criteria P/V Pg/V KLa tip rate mixing time 7.4. Scale-up CriteriaSize scale up7.4.1Scale up of siz

3、e of reactor Constant of air rate per volume（VVM1）（VVM2）（狀態(tài)）7.4.2Scale up of air input Constant of air rate per square area Constant of KLacorrection：改為：代入：VVMWgScale up methodbeforeafterbeforeafterVVm constant1113.33Wg constant10.311KLa constant10.51811.71 P/ Vconst 7.4.3 Scale up of power and spee

4、d of stirrer Pg/V=constant KLa =constant Stirrer reactor若 0.95 0.67 air lift reactorWtV power per volume Vtip=constantNRe P/V =constantT D diameter of tank and stirrer power per volume Tmix=constantConstant parameterStirrer speed in 10m3 vesselPower per volume (Pg/V)85Oxygen transfer (KLa)79Shear ra

5、te (NDi)50Mixing time (tm)1260Stirrer speed requirement when scaling up from a 10L STR to a 10m3 fermenter using different criteria7.5. Microbial responses to concentration gradients3.7L罐自制柵式槳 1500L罐特制攪拌槳 搖瓶發(fā)酵罐模擬計算平臺流場測試與分析工業(yè)化生產(chǎn)創(chuàng)新思路基于細胞群體效應(yīng)和傳遞特性的直接放大新思路 信號分子發(fā)酵罐內(nèi)流場分布發(fā)酵罐內(nèi)空氣分布1mm3mm5mm8mm10mm不同直徑的氣泡分布不同轉(zhuǎn)速和通氣量條件下流場的比較90rpm，1.5VVM105rpm，1VVM1mm90rpm，1.5VVM105rp