利用食品深加工技術(shù)分離制備大豆異黃酮Soybeanisoflavoneswereseparatedandpreparedbyfooddeepprocessingtechnology任務8-3Tasks8-3

綜合利用食品深加工技術(shù)分離制備大豆異黃酮Comprehensiveuseoffooddeepprocessingtechnologytoseparateandpreparesoybeanisoflavones二、設(shè)備與用具準備大豆豆粕、乙醇溶液。一、原料與試劑準備：取樣器、精密pH試紙、干燥箱、粉碎機、提取罐、離心機、組合超濾設(shè)備、D101大孔樹脂柱、層析柱、提取罐、儲罐、蒸發(fā)器、收集器、恒流泵、冷凍干燥機等。2.PreparationofequipmentandutensilsSoybeanmeal,ethanolsolution.1.Preparationofrawmaterialsandreagents:Sampler,precisionpHtestpaper,dryingoven,crusher,extractiontank,centrifuge,combinedultrafiltrationequipment,D101macroporousresincolumn,chromatographycolumn,extractiontank,storagetank,evaporator,collector,constantflowpump,freezedryer,etc.三、操作方法四、結(jié)果觀測分析樣品外觀現(xiàn)象檢測分析0天7天吸光值黃酮含量大豆異黃酮提取液

濃縮大豆黃酮提取液

純化大豆黃酮提取液

Fourth,theresultsofobservationandanalysissample外觀現(xiàn)象檢測分析0days7daysAbsorbancevalueFlavonoidcontentSoybeanisoflavoneextract

Concentratedsoybeanflavonoidextract

Purifiedsoybeanflavonoidextract

五、注意事項1.裝柱注意事項，請參照教材項目6的任務6-1相關(guān)內(nèi)容進行。2.選擇不同孔徑大小的膜對供試品溶液進行超濾，通過比較透析液平均流量超濾后溶液中大豆異黃酮的含量和各組分損失量以及雜質(zhì)的截留量來選擇適合的孔徑的膜。3.超濾壓力影響透過液的流量。通過比較超濾后雜質(zhì)的截留率和大豆異黃酮的透過率來選擇合適的超濾壓力。4.超濾膜在使用后進行有效的清洗是非常重要的，它可以保證處理物料的效果可靠與穩(wěn)定，延長膜的使用壽命，降低運行成本，因此使用之后用反沖法或者清水法進行清洗。5.Precautions1.PleaserefertoTask6-1ofitem6ofthetextbookforprecautionsforcolumnloading。2.MembraneswithdifferentporesizeswereselectedtoultrafilterthetestsolutionbycomparingthedialysateTheaverageflowrateisusedtoselectthemembranewithsuitableporesizeforthecontentofsoybeanisoflavonesinthesolutionafterultrafiltration,thelossofeachcomponentandtheretentionofimpurities.3.Ultrafiltrationpressureaffectstheflowrateofpermeate.Bycomparingtheretentionrateofimpuritiesafterultrafiltrationandthetransmittancerateofsoybeanisoflavonestoselecttheappropriateultrafiltrationpressure.4.Itisveryimportanttocarryouteffectivecleaningofultrafiltrationmembraneafteruse,whichcanensurethereliableandstableeffectofthetreatedmaterial,prolongtheservicelifeofthemembrane,andreducetheoperatingcost.一、實訓時間與分組安排二、實踐操作內(nèi)容的安排為了讓學生了解到不同超濾階段和超濾液的大豆異黃酮的含量差異，可以取不同時期的樣液測定大豆異黃酮的含量，更好的理解超濾技術(shù)對大豆異黃酮的分級濃縮的作用。1.本實訓任務宜對學生進行分崗位操作，崗位分別為：提取和離心、超濾、濃縮、層析、冷凍干燥。2.為了讓學生了解到不同環(huán)節(jié)的操作，可以讓學生輪流到不同崗位。3.如學校沒有成套的制備設(shè)備，可安排學生到相應的生產(chǎn)企業(yè)實習。1.Trainingtimeandgrouparrangement2.ArrangementofpracticaloperationcontentInordertoletstudentsunderstandthedifferencesinthecontentofsoybeanisoflavonesindifferentultrafiltrationstagesandultrafiltrates,thecontentofsoybeanisoflavonescanbedeterminedbytakingsamplesfromdifferentperiods,soastobetterunderstandtheeffectofultrafiltrationtechnologyontheclassificationandconcentrationofsoybeanisoflavones.1.Thetrainingtaskshouldbedividedintoposts,whichare:extractionandcentrifugation,ultrafiltration,concentration,chromatography,andfreeze-drying.2.Inorderforstudentstounderstandtheoperationofdifferentlinks,studentscantaketurnstodifferentpositions.3.Iftheschooldoesnothaveacompletesetofpreparationequipment,studentscanbearrangedtopracticeinthecorrespondingproductionenterprises.【技術(shù)拓展】LOGO將葛根粉碎，過60目，以50%乙醇為提取劑，料液比為1:8（g/mL），在70℃的環(huán)境下浸提2.5

h，離心、過濾，得葛根異黃酮粗提取液備用。將葛根異黃酮提取液，進行適當濃縮后，選用AB-8、HPD600、ADS-5等大孔樹脂進行分離純化，用95%乙醇溶液作為洗脫劑進行洗脫。取脫脂豆粕，用8~10倍75%的乙醇，60℃萃取4h，萃取液用20nm的無機陶瓷膜超濾，濃縮至質(zhì)量分數(shù)為30%，按醇料比7:1加入70%乙醇，攪拌10

min進行醇沉。上清液回收乙醇后調(diào)節(jié)其可溶性固形物含量為5%，靜置5

h，再以2000r/min速度離心10

min，對沉淀進行水洗，棄去水洗液，60℃的溫度下干燥沉淀物，得到純化大豆異黃酮粉末。一、利用大孔樹脂分離純化葛根異黃酮二、利用膜分離結(jié)合醇沉法純化大豆異黃酮【TechnologyExpansion】LOGOPuerarialobatawascrushed,passedthrough60mesh,50%ethanolwasusedastheextractionagent,thesolid-liquidratiowas1:8(g/mL),andtheextractionwascarriedoutat70°Cfor2.5h,centrifugedandfilteredtoobtainthecrudeextractofPuerarialobataisoflavonesforlateruse.AftertheisoflavoneextractofPuerarialobatawasproperlyconcentrated,AB-8,HPD600,ADS-5andothermacroporousresinswereselectedforseparationandpurification,and95%ethanolsolutionwasusedastheeluentforelution.Defattedsoybeanmealwasextractedwith8~10times75%ethanolat60°Cfor4h,theextractwasultrafilteredwithaninorganicceramicmembraneof20nm,concentratedtoamassfractionof30%,70%ethanolwasaddedaccordingtothealcohol-to-materialratioof7:1,andstirredfor10minforalcoholprecipitation.Afterthesupernatantrecoveredethanol,thesolublesolidscontentwasadjustedto5%,leftfor5h,centrifugedataspeedof2000r/minfor10min,theprecipitatewaswashedwithwater,theaqueouswashwasdiscarded,andtheprecipitatewasdriedatatemperatureof60°Ctoobtainpurifiedsoybeanisoflavonepowder.1.UsemacroporousresintoseparateandpurifyPuerariapirificaisoflavones2.Purificationofsoybeanisoflavonesbymembraneseparationcombinedwithalcoholprecipitation【學習思考】LOGO1.分離制備大豆異黃酮有何用途？2.在制備大豆異黃酮的過程中，最適宜的超濾膜具有什么特點？3.在使用大孔樹脂純化大豆異黃酮之前可否省掉濃縮步驟？【Learntothink】LOGO1.Whataretheusesofisolatingandprepari