INFLUENCESOFDIFFERENTDOSAGESANDDIFFERENTADMINISTRATIONROUTESONDRUGEFFECTS

INFLUENCESOFDIFFERENTDOSAGESONDRUGEFFECTS

PURPOSEToobservetheeffectsofpentobarbitalsodiumonmiceatdifferentdosage.PRINCIPLEPentobarbitalsodiumcandepressthecentralnervoussystemandproduceunconsciousnessorsleep.RightingreflexsleepingtimeMATERIALS1.Animal:3mice2.Drugs:0.2%,0.4%,0.8%pentobarbitalsodium3.Instruments:mousecage,balance,beaker,syringes(1ml),needlesMETHODS1.Weighandnumber3miceonebyone,thenobservetheirbehaviorandtherightingreflex.2.Administrate0.2%,0.4%,0.8%pentobarbitalsodiumatthedoseof20,40,80mg/kg(0.1ml/10g)tomouse#1,2,3byi.p.accordingly.Afteradministration,observetheirbehaviorandtherightingreflex,recordthesleepingtimeofeachmouse,andthenfillthedatainthetablebelow.RESULTSNOTESKeepquietandavoidshakingthemiceduringtheexperiment.QUESTIONS1.Whichgroupmiceproducetheshortestsleepingtimeafterthegivingpentobarbitalsodium?Whichgroupistheslowestone?Why?INFLUENCESOFDIFFERENTADMINISTRATIONROUTESONDRUGACTIONS

PURPOSEToobservetheinfluenceofdifferentadministrationrouteonpharmacologicalactionsofmagnesiumsulfate.PRINCIPLEFormostkindsofdrugs,differentadministrationroutesonlyaffecttheonset,strengthanddurationofdrugactioninsomeoccasions,thenatureofthedrugactionresultsindifferentpharmacologicaleffects.PRINCIPLEMagnesiumsulfate

whentakenorally,isnotsystemicallyabsorbedandactsasalaxativeforconstipation.whenitisinjected,magnesiumsulfateproducessystemicinhibitoryactionswhichincludecentralinhibition,hypotensionandmusclerelaxant.MATERIALS1.Animal:2mice2.Drugs:4%magnesiumsulfate3.Instruments:mousecage,balance,beaker,syringe(1ml),needleMETHODS1.Weighandnumber2miceonebyone,thenobservetheirbehavior.2.Administrate4%magnesiumsulfateatadoseof0.8g/kg(0.2ml/10g)tomouse#1,2byi.p.(intraperitonealinjection)andi.g.(intragastricinjection)respectively.3.Observethesymptomsofthemiceandrecordtheresultsinthefollowingtableaccordingly.RESULTSQUESTIONS1.W