1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemEHarringtonineCat. No.: HY-N0862CAS No.: 26833-85-2分式: CHNO分量: 531.59作靶點: Others作通路: Others儲存式: Powder -20C 3 years4C 2 yearsIn solvent -80C 6 months-20C 1 month溶解性數據體外實驗 DMSO : 100 mg/mL (188.11 mM)* means soluble, but saturatio

2、n unknown.Mass Solvent1 mg 5 mg 10 mg Concentration制備儲備液1 mM 1.8811 mL 9.4057 mL 18.8115 mL5 mM 0.3762 mL 1.8811 mL 3.7623 mL10 mM 0.1881 mL 0.9406 mL 1.8811 mL請根據產品在不同溶劑中的溶解度，選擇合適的溶劑配制儲備液，并請注意儲備液的保存式和期限。體內實驗請根據您的實驗動物和給藥式選擇適當的溶解案，配制前請先配制澄清的儲備液，再依次添加助溶劑(為保證實驗結果的可靠性，體內實驗的作液，建議您現現配，當天使；澄清的儲備液可以根據儲存條件，適

3、當保存；以下溶劑前的百分 指該溶劑在您配制終溶液中的體積占)：1. 請依序添加每種溶劑： 10% DMSO 40% PEG300 5% Tween-80 45% salineSolubility: 2.5 mg/mL (4.70 mM); Clear solution2. 請依序添加每種溶劑： 10% DMSO 90% (20% SBE-CD in saline)Solubility: 2.5 mg/mL (4.70 mM); Clear solution3. 請依序添加每種溶劑： 10% DMSO 90% corn oil1/2 Master of Small Molecules 您邊的抑制

4、劑師www.MedChemESolubility: 2.5 mg/mL (4.70 mM); Clear solutionBIOLOGICAL ACTIVITY物活性 Harringtonine種天然的 Cephalotaxus 物堿，可抑制蛋質合成。體外研究 Harringtonine inhibits the elongation phase of translation by preventing substrate binding to the acceptor siteon the 60-S ribosome subunit and therefore block aminoacyl

5、-tRNA binding and peptide bond formation 1.Harringtonine displays potent inhibition of Chikungunya virus infection with an EC50 of 0.24 M.Harringtonine could inhibit other alphaviruses 2. Harringtonine inhibits the growth of human myeloidleukemia cells in vitro at low concentrations. The mechanism o

6、f the antitumor action of harringtonine isconsidered to be an effect on protein synthesis and is characterized by breakdown of polysomes tomonosomes 3.PROTOCOLCell Assay 2 For harringtonine treatment studies with Sindbis virus, BHK21 cells are seeded into 96-well plates andinfected with Sindbis viru

7、s at an MOI of 1 for 1 h prior to being washed twice with PBS and incubated withvarious concentrations of harringtonine (0.1 M, 1 M, 5 M, and 10 M) at 37C with 5% CO2. Cellsupernatants are harvested for plaque assays at 24 h postinfection 2.MCE has not independently confirmed the accuracy of these m

8、ethods. They are for reference only.戶使本產品發表的科研獻 Nucleic Acids Res. 2019 Jan 31.See more customer validations on HYPERLINK / www.MedChemEREFERENCES1. Fresno M, et al. Inhibition of translation in eukaryotic systems by harringtonine. Eur J Biochem. 1977 Jan;72(2):323-30.2. Kaur P, et al. Inhibition of

9、 chikungunya virus replication by harringtonine, a novel antiviral that suppresses viral protein expression.Antimicrob Agents Chemother. 2013 Jan;57(1):155-67.3. Piao YF, et al. Growth inhibition of human myeloid leukemia cells in vitro by harringtonine. Gan To Kagaku Ryoho. 1990 Feb;17(2):281-5.McePdfHeightCaution: Product has not been fully validated for medic