1、PTR-MS & Quadrupole ion guide OutlineuPTR-MSuQuadrupole ion guideuNext workPTR-MSlIntroduction Overview Development Applicationl Component Ion Source Drift Tube Quadrupole mass analyzer？PTR-MS李建權等.質子轉移反應質譜的建立與性能研究J.分析化學,2008,36(1)細胞檢測器ATP質譜圖OverviewPTR-MSDevelopment1966:F.H.Field and M.S.B.Munso

2、n developed chemical ionization;1969:Flowing afterglow and flow drift tube technique (FDT) developed by Ferguson and colleagues ;1990s:On the basis of SIFT-MS,Innsbrukes Lindinger team put forward the PTR-MS technology for the first time in combination with chemical ionization theory (1996) and drif

3、t tube model (1969).In June of 1995, Lindinger: Acetonitrile and benzene in the breath of smokers and non-smokers investigated by PTR-MS;In July of 1995, Lindinger: PTR-MS: Online Trace Gas Analysis at ppb Level.1998,the first commercial PTR-MS instrument was produced by IONICON Analytik.Schematic o

4、f PTR-TOF-MSBlake R S, Whyte C, Hughes C O, et al. Demonstration of proton-transfer reaction time-of-flight mass spectrometry for real-time analysis of trace volatile organic compounds.J. Analytical Chemistry, 2004, 76(13):3841-5.Hansel A,et al. Proton-transfer-reaction mass spectrometry (PTR-MS): o

5、n-line monitoring of volatile organic compounds at volume mixing ratios of a few pptvJ. Plasma Sources Science & Technology, 1999, volume 8(2):332-336(5).Schematic of PTR-MS PTR-MSDevelopmentHigh sensitivity(a few pptv) Multichannel data acquisitionApplicationsl Air quality testing (Real-time on

6、line quantitative measurements of trace VOCs)l Biological research (Monitor the response of plant leaf wounds)l Food science and aroma industry(Research on aroma, Production process control)l Disease diagnosis (Lung cancer,Rectal cancer,diabetes)PTR-MSIon Source (H3O+ Generator)伍小梅等.在線檢測揮發性有機物的質子轉移反

7、應飛行時間質譜儀的研制J.質譜學報,2015,36(1)Structure of Double Hollow Cathode DischargeDrift Tube (Proton transfer reaction)Structure of Drift Tube李建權,沈成銀,王鴻梅等.質子轉移反應質譜的建立與性能研究J.分析化學,2008,36(1)32VOCsH OVOCsHH OQuadrupole mass analyzera. Hyperboloidb. Cylinder有效區域四極場作用區域Machining difficulty：abMachining accuracy：baF

8、ree degree：r/r0 r=1.148r0(Deviation 1 % )直流分量: u交流分量 :V cos t場半徑: r0令= 1/2t Mathieu方程 Quadrupole mass analyzer方向,覃莉莉,白崗等.四極桿質量分析器的研究現狀及進展J.質譜學報,2005,26(4)Mathieu參數 Quadrupole mass analyzerStability of Solution of Mathieu EquationQuality scan line:y=kx(k=a/q=2U/V) Changing the values of U and V can a

9、chieve quality scanning.The movement of ions in the quadrupole can be described by the solution of the Mathieu equation.First stable region方向,覃莉莉,白崗等.四極桿質量分析器的研究現狀及進展J.質譜學報,2005,26(4)Quadrupole mass analyzerMiller P E. Investigations of the RF-only quadrupole mass analyzer J. Dissertations & The

10、ses, 1985.m+1:passm,m+2:cant passm+1, m+2:passm:cant passChanging the slope of the quality scan line (K = a/q), that is, changing the u/v value causes the scan line to move down.What will happen if K = 0 (u = 0)?OutlineuPTR-MSuQuadrupole ion guideuNext workQuadrupole ion guide (RF-only ion guide)Why

11、 do we want to use quadrupole ion guide?l Reduce the loss of ions in the transmission pathl Reduce the space divergence and energy dispersion of ionsl Improve sensitivityl Transmission efficiency is highl Requirement of vacuum degree is lowQuadrupole ion guide (RF-only ion guide)When u = 0, the mass

12、 scan line coincides with the q axis, theoretically, all the ions in the stable region can pass through the quadrupole ion guide device.黃超. 基于頻率掃描的數字化四極桿濾質器的研究D. 天津大學, 2007.2 LINAC 式1.Guo, et al. A radio frequency signal driver for quadrupole used in desktop orthogonal-injection time-of-flight mass

13、spectrometer J. Nuclear Instruments & Methods in Physics Research, 2012, 694(6):255-262.2.3.4.黃正旭.高分辨垂直引入式飛行時間質譜儀原理及設計.D.廣州.中科院廣州地球化學研究所，2009 134Quadrupole ion guide (RF-only ion guide)Quadrupole ion guide (RF-only ion guide)Probulems must be solvedl Working air pressure of quadrupole ion guide-

14、design of the vacuum system (selection of pump, Cavity size, opening size.)l Parameter estimation and mechanical design of Quadrupolel Design of matching circuitl Low-mass cut-off value (LMCO) l Test and modifyOutlineuPTR-MSuQuadrupole ion guideuNext workNext workl Learn how to use SIMIONl Define the energy, angle, and position of random ionsl Define the parameters of the quadrupole transmission (voltage, physical dimension.)N