精品文檔-下載后可編輯腦外傷后抑郁狀態模型的建立及效果評價【摘要】目的探討建立腦外傷后抑郁狀態動物模型，并對此模型的建立和有效性做系統評價。方法本實驗在武漢大學醫學院實驗中心完成。選用48只雄性Wistar大鼠，隨機(隨機數字法)分為4組，每組12只。①假手術組：每籠6只飼養，正常飲食飲水；②模型組：以自由落體撞擊法制作大鼠左側額葉腦挫裂傷模型，每籠1只飼養，術后1周加以慢性應激和孤養方法，造成腦外傷后抑郁狀態動物模型；③腦外傷組：以自由落體撞擊法制作大鼠左側額葉腦挫裂傷模型，每籠6只飼養，正常飲食飲水；④慢性不可預見性溫和應激組：每籠1只飼養，采用慢性應激方法結合孤養法造成大鼠慢性應激抑郁模型。觀察各組大鼠蔗糖水試驗，自發改變，用高效液相色譜熒光檢測法測定各組大鼠腦內海馬、前額葉、下丘腦和紋狀體不同部位的多巴胺、5-羥色胺、去甲腎上腺素單胺類神經遞質含量。采用SPSS13.0統計軟件分析結果，資料采用單因素方差分析或成組t檢驗，以P<0.05為差異具有統計學意義。結果造模后即予行為學測試，模型組大鼠蔗糖水飲用量逐漸減少，反映模型組大鼠興趣和缺乏；曠野試驗中模型組大鼠水平及垂直得分減少，反映模型組大鼠活動性減少，興趣缺乏；模型組大鼠游到終點的時間延長，錯誤次數增加，反映模型組大鼠的學習和記憶能力下降；模型組大鼠海馬、前額葉、下丘腦，和紋狀體多巴胺、5-羥色胺、去甲腎上腺素含量下降。結論在左側額葉腦挫裂傷模型的基礎上給予孤養和慢性不可預見的溫和性應激，能建立較理想的腦外傷后抑郁狀態動物模型。

【關鍵詞】腦外傷；抑郁；動物模型;單胺類神經遞質;形態學

EstablishmentandevaluationofmodelofdepressionaftertraumaticbraininjuryCHUSheng-hua,CHENEr-tao,FENGDong-fu,MAYan-bin,ZHUZhi-an.DepartmetofNeurosurgery,NO.省略

【Abstract】ObjectiveTodiscussabouttheestablishmentofmentaldepressionmodelbytraumaticbraininjuryinrats，andtoevaluatethevalidityofthismodel.MethodsThestudywascarriedoutintheexperimentcenterofWuhanUniversity.Atotalof48maleWistarratswererandomized（randomnumber）intofourgroups(n=12,each):(1)inshamoperationgroup,sixratswerefednormallyinonecage;(2)inmodelgroup,themodeloftheleftfrontallobecontusionwasmadeupbyhittingwithfree-fallmethodontheleftcortexoftherat,andsubsequentlytheeachofmodelratswereseparatelyfedinacageandputtheminalonelyenvironmentwithchronicstressoneweekaftertraumaticbraininjuryinordertoinducethemintomodelsofmentaldepression;(3)inbraintraumagroup,thetraumamodeloftheleftfrontallobecontusionwassetupbythesameprocedureasinmodelgroup,andsixratswerefedtogetherinonecage;(4)inCUMSgroup,eachratwasfednormallyinonecageinalonelyenvironmentafterbraintraumamadeandchronicmildchaoticstressunpredictabletoratswasgiventoinducementaldepression.Theconsumptionofsucrosewaterandthechangeofanimalbehaviorwereobservedandthehighperformanceliquidphaseelectrochemicalwithfluorescencedetectorwasusedtodetectthebiogenicmonoamineneurotransmittercontent(dopamine,serotoninandnorepinephrine)inthehippocampus,prefrontalcortex,hypothalamusandstriatumareaineachgroup.ExperimentaldatawereprocessedwithsinglefactoranalysisorttestofvariancebyStatisticalProgramforSocialSciencesVersion13.0(SPSS13.0)software.P<0.05wasconsideredasstatisticallysignificantdifference.ResultsAftermodeling,theratsoffourgroupswereexaminedbybehavioraltests.Itwasfoundthattheamountofsugar-waterconsumptionbytheratsinthemodelgroupandCUMPgroupdecreased,implyingtheabsenceofinterestineatingandanhedoniaoftheratsinthesegroups.Inopen-fieldtest,theratsinthemodelgroup,CUMPgroupandbraintraumagroupshowedreductioninsquarecrossingandrearing,implyingtheunder-activityandabsenceofinterestinactivity.Inthewatermazetest,itwasfoundthattheratsinthemodelgroup,CUMPgroupandbraintraumagroupneededmuchtimetoreachtheend-pointandmademanyerrors,implyingtheloweringabilitytolearnandmemorize.Levelsofdopamine,serotoninandnorepinephrineinthehippocampus,prefrontalcortex,hypothalamusandstriatumareadecreasedintheseratswithtraumatizedbraininthreegroupsespeciallyinmodelgroupandCUMPgroup.ConclusionsItisavalidmethodforestablishingthementaldepressionmodelinrat