1、Miranda D. Grounds, et al. The Journal of Histochemistry & Cytochemistry 2019,50(5): 589610 BMC(bone marrow cell)Nature/Vol428/3 April 2019/664-8 Problems of HSCHSCCD34+Transplatition for AMIJ Thorac Cardiovasc Surg 2019;130:1631-8 骨髓間充質(zhì)干細(xì)胞mesenchymal stem cell, MSC K.D. Bunting, R.G. Hawley .Biolog

2、y of the Cell 2019,95:563578MSC(mesenchymal stem cell) DIFFERITIONStem cells from bone m arrow could be used to repair a variety of tissues.？原代傳代前細(xì)胞形狀原代傳代前細(xì)胞形狀100 貼壁細(xì)胞增殖交融，鋪滿貼壁細(xì)胞增殖交融，鋪滿瓶底瓶底90% 左右，未貼壁左右，未貼壁細(xì)胞是造血干細(xì)胞及組織細(xì)胞是造血干細(xì)胞及組織碎片碎片p1100 p4100 p3100 p2100 CD106 、CD90表達(dá)陽性，表達(dá)陽性， CD31、CD45表達(dá)陰性表達(dá)陰性AMINor

3、mal復(fù)方丹參滴丸對(duì)骨髓間充質(zhì)干細(xì)胞移植治療急性心肌梗死干涉作用的實(shí)驗(yàn)研討復(fù)方丹參滴丸對(duì)骨髓間充質(zhì)干細(xì)胞移植治療急性心肌梗死干涉作用的實(shí)驗(yàn)研討Normal AMINBTNORMAL AMIAMI Group左室后壁增厚，運(yùn)動(dòng)幅度變小 CDDPMSC Group左室后壁厚度均勻，運(yùn)動(dòng)幅度正常0102030405060708090正常組模型組G-CSF組復(fù)方丹參滴丸組MSCs移植組MSCs移植+G-CSF組MSCs移植+復(fù)方丹參滴丸組EF對(duì)AMI大鼠心臟射血分?jǐn)?shù)的影響孫蘭軍等，中華醫(yī)學(xué)臨床雜志，2019，62：4模型組MSCs復(fù)方丹參滴丸組極少量移植地細(xì)胞能表達(dá)-actinin，闡明開場(chǎng)向心肌細(xì)胞

4、分化，但這種分化是不完全的，由于沒有橫紋的出現(xiàn)。相對(duì)較多的細(xì)胞表達(dá)血管性血友病因子(vWF) ，闡明移植的MSC可以向內(nèi)皮細(xì)胞分化。在同一視野看到在同一視野看到Dil標(biāo)志的植入的紅色熒光的標(biāo)志的植入的紅色熒光的MSCs和二抗和二抗FITC標(biāo)志的相應(yīng)綠色熒光的標(biāo)志的相應(yīng)綠色熒光的 CX-43陽性表達(dá)陽性表達(dá)CDDP+MSC組組200MSC組組200CDDP+MSC組組200 在同一視野看到在同一視野看到Dil標(biāo)志的植入的紅色熒光的標(biāo)志的植入的紅色熒光的MSCs和二抗和二抗FITC標(biāo)標(biāo)志的相應(yīng)綠色熒光的志的相應(yīng)綠色熒光的 Troponin 陽性表達(dá)，陽性表達(dá)，MSCs Group Troponin

5、 表達(dá)表達(dá)200 HE Picrosirius red HE Picrosirius red normalMSCs+CDDPMSCs model各心梗組大鼠的膠原含量無顯著差別。但是，MSC和CDDP+MSC移植使心肌梗死區(qū)I/III型膠原比值分別降低了77和84。闡明細(xì)胞移植能添加III型膠原含量，改善心肌順應(yīng)性，能夠會(huì)對(duì)心功能有益。050100150200250300controlm odelbai cal i nMigration (% of control)*Balcalin induced migration of Mscs00. 511. 522. 50h6h12h18h24hVE

6、GF gene expression00. 511. 522. 50h6h12h18h24hMCP-1 gene expression00. 511. 522. 530h6h12h18h24hSCF gene expression* p 0.05, * p 0.01, compared to model. (n=6), *020406080100120bai cal i nbai cal i n/anti -VEGFbai cal i n/anti -M CP-1Migration (% of baicalin)*Randomized Clinical Trials of Stem Cell Therapy for MICirculation. 2019;114:353-358. Arrhythmias Restenosis, Accelerated Atherosclerosis, and Coronary Obstruction Abnormal Cellular Differenti